FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004965470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183319
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN720196.086186396060572No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG71320.60271152580158No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC71270.6022889854722183No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG44440.3755538447367109No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG36950.31225730339832286No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG36310.30684878718249264No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG32550.2750737544144901No Hit
GCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCG29740.25132698790436053No Hit
GAGGGGACAGATGTGGTCCCTCAGCCTGGGGGGACAAGTGTGGCTCCTC25630.21659417283082585No Hit
ATAATAATTCTTACCATTGACTTTCACAGTAATTCGCTTGTAGTCGGCAC24850.21000254369278273No Hit
GAGGAGCCACACTTGTCCCCCCAGGCTGAGGGACCACATCTGTCCCCTC24350.2057771403991654No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT23830.20138272097380336No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG23480.19842493866827118No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT23440.1980869064047818No Hit
CCCGCAGGCGGGAGTAGCAGTGGTTCATGTCGTCCAGCAAGCTCAGCGGCTCCTC21250.17957963997873777No Hit
GAGGAGCCGCTGAGCTTGCTGGACGACATGAACCACTGCTACTCCCGCCTGCGGG21150.1787345593200143No Hit
CTAAGCAATTAGTACAAACACCACAGTCCTCAGGCACCTGGCAGCCGGGACACTGCCC20260.1712133414573754No Hit
GGGCAGTGTCCCGGCTGCCAGGTGCCTGAGGACTGTGGTGTTTGTACTAATTGCTTAG19740.16681892203201334No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA18840.1592131961035021No Hit
GACCACCTCGATGAGGTTGGCCTGCAGGTACAGGTACTCCAGCTTGCCC18540.15667795412733168No Hit
GGGCAAGCTGGAGTACCTGTACCTGCAGGCCAACCTCATCGAGGTGGTC18270.1543962363487783No Hit
GGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCT17460.1475510830131182No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG17380.1468750184861394No Hit
AAGGAAATACTACCTTTTCTTAAACGGAAGATGAATGTCAGTGCTACACC17340.14653698622265002No Hit
CAAGACCCTGGAGGGCGAGTTCTCGGTCACCATGTGGTCCTCAGGTGAGC16660.14079043774333042No Hit
CAAAACAAAAACATCCCGGGGCCGGGGCGCGCGGCCGGCGCTCACCTGAG16310.13783265543779827No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG15900.13436782473703202No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA15720.13284667955132978No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT15130.1278607036648613No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC14600.1233817761736269No Hit
GCCGAGTCCCCACTGGCCACCATGTGGGGCACGCAGGAGC14190.11991694547286065No Hit
GGAGAGGAACGACTGGCATCCCATAATTTATTTCGGGAGGAGGAACAGTG13860.11712817929907321No Hit
CCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAA13840.1169591631673285No Hit
CCGCGTTGTCAAGGACAAGATCGGCGAGGAGCAGCGCAAGACCATCTGGG13700.11577605024511566No Hit
CACAGTAGGTGATCCTTTACTCTCCTCTGGACTTCGAAGCATTGGCTCCA13680.11560703411337095No Hit
CGAAGTCCGTGTAGACGGTGTCCTTGAGCAGCGCCCCCGACCCGAAGTCG13610.11501547765226453No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT13550.11450842925703043No Hit
CATCAACACCATCCAACTGTGAGATTTTTGGAAGATCACACTGTTCCTCC13480.11391687279592401No Hit
GGTGGTGGTGGTCTTACCACTCAGGACTTGATGGTCACTGCTTGTCCAGA13190.11146613888562594No Hit
GCCCGCCTCACAGCTGGTCCTCGTCCACCCAGATGGTCTTGCGCTGCTCC13100.11070556629277481No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC12890.10893089690945552No Hit
GGCACAAGCTCAGGCCGCAGACGGTGCCGGCCCCGAGGACGACACTGAGG12780.1080013081848597No Hit
GGACCGCTGGGGTGATAAGGAAGAGGTACTGTGACGCCTGGAGCCAATGC12740.1076632759213703No Hit
TGTCATCCACGTCCTGGAGCCAGGAGATGAACTGGCGGGCGTTGAAACGG12610.10656467106502981No Hit
GCTCCTGCGTGCCCCACATGGTGGCCAGTGGGGACTCGGC12520.10580409847217868No Hit
CCGGCTGGATGCCGCCAGGAGGGCCTTCGGCTGGGGCGGCGGCACGGGAG12480.10546606620868929No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG12470.10538155814281694No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT12440.1051280339451999No Hit
CAAGGACGAAAACATCCTTATCGACCTCAATCGCGGCGAGCTCAAGCTCA12290.10386041295711468No Hit
CAGGTGGTGGTGACAGGCGTGATGCCTCAGGTGGTGGGG12230.10335336456188061No Hit
TGCTCTGCAGGTAATCATCAGAGACAACCACCACCATCCGGCGGCACCTA12210.10318434843013592No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG12130.10250828390315712No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG12120.10242377583728479No Hit
CCCCACCACCTGAGGCATCACGCCTGTCACCACCACCTG11890.10048009032222081No Hit
CCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAA11880.10039558225634845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)