FastQCFastQC Report
Wed 8 May 2024
EGAF00004965471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183319
Total Bases112 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN708885.990607773558947No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC69990.5914719530405579No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG69520.5875000739445576No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG43810.370229836586753No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG35830.30279240002061997No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG35730.3019473193618965No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG31890.26949622206691515No Hit
GCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCG30240.2555523911979779No Hit
CCCGCAGGCGGGAGTAGCAGTGGTTCATGTCGTCCAGCAAGCTCAGCGGC26970.22791825365772034No Hit
GAGGAGCCACACTTGTCCCCCCAGGCTGAGGGACCACATCTGTCCCCTC24850.21000254369278273No Hit
CTAAGCAATTAGTACAAACACCACAGTCCTCAGGCACCTGGCAGCCGGGA24640.2082278743094635No Hit
ATAATAATTCTTACCATTGACTTTCACAGTAATTCGCTTGTAGTCGGCAC24640.2082278743094635No Hit
GAGGGGACAGATGTGGTCCCTCAGCCTGGGGGGACAAGTGTGGCTCCTC24390.20611517266265478No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT24330.2056081242674207No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT23580.19927001932699465No Hit
GGGCAGTGTCCCGGCTGCCAGGTGCCTGAGGACTGTGGTGTTTGTACTAA23320.19707280961431367No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG23270.19665026928495188No Hit
GAGGAGCCGCTGAGCTTGCTGGACGACATGAACCACTGCTACTCCCGCCT23130.19546715636273904No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGC19100.16141040581618313No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA18520.156508937995587No Hit
GGGCAAGCTGGAGTACCTGTACCTGCAGGCCAACCTCATCGAGGTGGTC18420.1556638573368635No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG18370.15524131700750177No Hit
GACCACCTCGATGAGGTTGGCCTGCAGGTACAGGTACTCCAGCTTGCCC17940.15160747017499085No Hit
AAGGAAATACTACCTTTTCTTAAACGGAAGATGAATGTCAGTGCTACACC17760.1500863249892886No Hit
GGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCT17120.14467780877345837No Hit
CAAGACCCTGGAGGGCGAGTTCTCGGTCACCATGTGGTCCTCAGGTGAGC16340.13808617963541528No Hit
CAAAACAAAAACATCCCGGGGCCGGGGCGCGCGGCCGGCGCTCACCTGAG16010.13529741346162785No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA15560.13149455049737221No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG15500.13098750210213814No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC14850.12549447782043557No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT14750.1246493971617121No Hit
GGAGAGGAACGACTGGCATCCCATAATTTATTTCGGGAGGAGGAACAGTG13980.11814227608954136No Hit
GGTGGTGGTGGTCTTACCACTCAGGACTTGATGGTCACTGCTTGTCCAGA13880.1172971954308179No Hit
CCGCGTTGTCAAGGACAAGATCGGCGAGGAGCAGCGCAAGACCATCTGGG13760.11628309864034972No Hit
GCTCCTGCGTGCCCCACATGGTGGCCAGTGGGGACTCGGC13620.11509998571813686No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT13570.11467744538877513No Hit
GGACCGCTGGGGTGATAAGGAAGAGGTACTGTGACGCCTGGAGCCAATGC13440.11357884053243461No Hit
CATCAACACCATCCAACTGTGAGATTTTTGGAAGATCACACTGTTCCTCC13370.11298728407132819No Hit
CACAGTAGGTGATCCTTTACTCTCCTCTGGACTTCGAAGCATTGGCTCCA13240.11188867921498767No Hit
CAAGGACGAAAACATCCTTATCGACCTCAATCGCGGCGAGCTCAAGCTCA13130.11095909049039186No Hit
GCCCGCCTCACAGCTGGTCCTCGTCCACCCAGATGGTCTTGCGCTGCTCC13080.11053655016103012No Hit
CCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAA12880.10884638884358319No Hit
CCGGCTGGATGCCGCCAGGAGGGCCTTCGGCTGGGGCGGCGGCACGGGAG12820.1083393404483491No Hit
CGAAGTCCGTGTAGACGGTGTCCTTGAGCAGCGCCCCCGACCCGAAGTCG12720.10749425978962561No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC12690.10724073559200857No Hit
GCCGAGTCCCCACTGGCCACCATGTGGGGCACGCAGGAGC12430.10504352587932755No Hit
CAACATTTTAATTAAAATGATTTACCTTAACGAAGTCTCTATCAGTCTTC12320.10411393715473174No Hit
GGCACAAGCTCAGGCCGCAGACGGTGCCGGCCCCGAGGACGACACTGAGG12300.10394492102298704No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG12260.10360688875949765No Hit
TGTCATCCACGTCCTGGAGCCAGGAGATGAACTGGCGGGCGTTGAAACGG12240.10343787262775295No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG12210.10318434843013592No Hit
GCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTG12140.10259279196902948No Hit
GCTCGTGGTAGAGGTCCAGCTTATTGTGGGACAGGTCTAGCACCTGCAGA12080.10208574357379539No Hit
ACTAATTGCTTAGATAAGCCCAAGTTTGGTGGTCGCAATATAAAGAAGCA12060.1019167274420507No Hit
GTCCACAGTGGGGGTCCGGTGCGGCGTTGTGCTCTCTGTAACTGCGGGGA12050.10183221937617837No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG11940.10090263065158254No Hit
TGCTCTGCAGGTAATCATCAGAGACAACCACCACCATCCGGCGGCACCTA11940.10090263065158254No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT11920.10073361451983784No Hit
CCTCTTATGCAGAGCCCCAGTACTTTCGCTTCGAAGGTGTGTGGCTGACA11920.10073361451983784No Hit
CCCCACCACCTGAGGCATCACGCCTGTCACCACCACCTG11910.1006491064539655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)