FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004965471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183319
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN708885.990607773558947No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC69990.5914719530405579No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG69520.5875000739445576No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG43680.3691312317304125No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG35830.30279240002061997No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG35730.3019473193618965No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG31850.26915818980342576No Hit
GCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCG30200.2552143589344885No Hit
GAGGAGCCACACTTGTCCCCCCAGGCTGAGGGACCACATCTGTCCCCTC24850.21000254369278273No Hit
ATAATAATTCTTACCATTGACTTTCACAGTAATTCGCTTGTAGTCGGCAC24610.20797435011184642No Hit
GAGGGGACAGATGTGGTCCCTCAGCCTGGGGGGACAAGTGTGGCTCCTC24390.20611517266265478No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT24290.20527009200393131No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT23530.19884747899763291No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG23240.19639674508733487No Hit
GAGGAGCCGCTGAGCTTGCTGGACGACATGAACCACTGCTACTCCCGCCTGCGGG21080.17814300285890786No Hit
CCCGCAGGCGGGAGTAGCAGTGGTTCATGTCGTCCAGCAAGCTCAGCGGCTCCTC20590.17400210763116286No Hit
GGGCAGTGTCCCGGCTGCCAGGTGCCTGAGGACTGTGGTGTTTGTACTAATTGCTTAG19600.1656358091098005No Hit
CTAAGCAATTAGTACAAACACCACAGTCCTCAGGCACCTGGCAGCCGGGACACTGCCC19420.16411466392409824No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA18460.1560018896003529No Hit
GGGCAAGCTGGAGTACCTGTACCTGCAGGCCAACCTCATCGAGGTGGTC18420.1556638573368635No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG18320.15481877667814004No Hit
GACCACCTCGATGAGGTTGGCCTGCAGGTACAGGTACTCCAGCTTGCCC17940.15160747017499085No Hit
AAGGAAATACTACCTTTTCTTAAACGGAAGATGAATGTCAGTGCTACACC17760.1500863249892886No Hit
GGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCT17100.14450879264171368No Hit
CAAGACCCTGGAGGGCGAGTTCTCGGTCACCATGTGGTCCTCAGGTGAGC16320.13791716350367061No Hit
CAAAACAAAAACATCCCGGGGCCGGGGCGCGCGGCCGGCGCTCACCTGAG15980.1350438892640108No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA15540.13132553436562752No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG15470.1307339779045211No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC14810.1251564455569462No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT14720.12439587296409506No Hit
GGAGAGGAACGACTGGCATCCCATAATTTATTTCGGGAGGAGGAACAGTG13950.11788875189192433No Hit
GGTGGTGGTGGTCTTACCACTCAGGACTTGATGGTCACTGCTTGTCCAGA13840.1169591631673285No Hit
CCGCGTTGTCAAGGACAAGATCGGCGAGGAGCAGCGCAAGACCATCTGGG13730.11602957444273268No Hit
GCTCCTGCGTGCCCCACATGGTGGCCAGTGGGGACTCGGC13620.11509998571813686No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT13510.11417039699354103No Hit
GGACCGCTGGGGTGATAAGGAAGAGGTACTGTGACGCCTGGAGCCAATGC13410.11332531633481757No Hit
CATCAACACCATCCAACTGTGAGATTTTTGGAAGATCACACTGTTCCTCC13300.11239572761022176No Hit
CACAGTAGGTGATCCTTTACTCTCCTCTGGACTTCGAAGCATTGGCTCCA13180.1113816308197536No Hit
CAAGGACGAAAACATCCTTATCGACCTCAATCGCGGCGAGCTCAAGCTCA13130.11095909049039186No Hit
GCCCGCCTCACAGCTGGTCCTCGTCCACCCAGATGGTCTTGCGCTGCTCC13050.11028302596341308No Hit
CCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAA12870.10876188077771083No Hit
CCGGCTGGATGCCGCCAGGAGGGCCTTCGGCTGGGGCGGCGGCACGGGAG12820.1083393404483491No Hit
CGAAGTCCGTGTAGACGGTGTCCTTGAGCAGCGCCCCCGACCCGAAGTCG12720.10749425978962561No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC12670.10707171946026388No Hit
GCCGAGTCCCCACTGGCCACCATGTGGGGCACGCAGGAGC12430.10504352587932755No Hit
GGCACAAGCTCAGGCCGCAGACGGTGCCGGCCCCGAGGACGACACTGAGG12300.10394492102298704No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG12240.10343787262775295No Hit
TGTCATCCACGTCCTGGAGCCAGGAGATGAACTGGCGGGCGTTGAAACGG12180.10293082423251888No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG12160.10276180810077419No Hit
GCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTG12130.10250828390315712No Hit
GCTCGTGGTAGAGGTCCAGCTTATTGTGGGACAGGTCTAGCACCTGCAGA12070.10200123550792306No Hit
GTCCACAGTGGGGGTCCGGTGCGGCGTTGTGCTCTCTGTAACTGCGGGGA12040.10174771131030601No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG11930.1008181225857102No Hit
CCCCACCACCTGAGGCATCACGCCTGTCACCACCACCTG11910.1006491064539655No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT11890.10048009032222081No Hit
CCTCTTATGCAGAGCCCCAGTACTTTCGCTTCGAAGGTGTGTGGCTGACA11890.10048009032222081No Hit
ACTAATTGCTTAGATAAGCCCAAGTTTGGTGGTCGCAATATAAAGAAGCA11870.10031107419047611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)