FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237734
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN126020.5631589813623961No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG106740.47700039414872364No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT72730.3250162887992943No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG71370.3189387121078734No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG62990.281490114553383No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG59300.26500021897151316No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG55820.24944877273170094No Hit
AGAAAGGGGTTTGTGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCAC47520.21235767968847055No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG47170.21079359745170786No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT46490.20775480910599742No Hit
CTGTAGGAAGAGACACCTCTGTTTCTTGCTGCCCGGGAGGGGAGCTATGA45770.20453726850465695No Hit
GTTTAGGTTTCAAAGGTGTGCATTGTGAATTAGAAATAAATGAATGTCAG45540.20350944303478427No Hit
GTGATGTCTCGATTGGCAAAATGGTCTAACAGGATCTTGGCTGCTTCATA45220.2020794249897441No Hit
CAAAGCGCCGTAGCTCCCGCTGCCCGTGTAGACTGGGCTCCCCGCAGTTA45170.20185598467020657No Hit
GTCCACAGTGGGGGTCCGGTGCGGCGTTGTGCTCTCTGTAACTGCGGGGA44550.19908532470794116No Hit
CGATACACTTTGCCCCATTCAGACACGGAGTACTGGAACAGTCATCAATA44040.19680623344865833No Hit
GTTTTCTCTGCAGTCTTTAAACCAGAGGAGTTACGCCAGGCCCTCATGCC44020.1967168573208433No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC43420.19403557348639294No Hit
AGCAGCACCAGCAATCTCAAGACCCACCTGCGACTCCATTCTGGAGAGAA43300.19349931671950285No Hit
GACCACACACCTTGGCTTATGCCTCCAACAATCCCCTTAGCATCACCATT43030.19229273899400018No Hit
AGATTTTCGCTTCCCTTGCATAGGAGCAGTGGAAGCAGGCAAAAATGGTG42440.1896561432234573No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC41700.18634922649430183No Hit
CATCAACACCATCCAACTGTGAGATTTTTGGAAGATCACACTGTTCCTCC41380.18491920844926163No Hit
ATCCACACACTGCCCATTGTTCACACAAGGGTTGCTCTGACATTCATTTA41290.18451701587409408No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA41170.183980759107204No Hit
CTGGGTCCTGTCGATACAGTGCTTCTAGGGTTGGCATGAGGGCCTGGCGT40700.18188042010355118No Hit
ATCGCCTCTATGTGTCTGTGAAGGAGATTGGTGGATTGACTCAGGTGAGT40330.18022696173897343No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT40040.17893100788565575No Hit
TTTGAGGTTTCACTGGGCCAGTTTGCCAGATTGATATTGATGACTGTTCC39880.17821599886313566No Hit
CAAGAGGCACACGCTGCAGACCCACAGTGACAAACCCTACAAGTGTGACC39210.17522189858133272No Hit
GGAGAGGAACGACTGGCATCCCATAATTTATTTCGGGAGGAGGAACAGTG39160.1749984582617952No Hit
CAAATGTTATCTGTGAAAAGGCAGCAACAGCAGCAACATTCACCAGCACC39130.17486439407007268No Hit
GGGTGAACTTGGCAGGGCACACCTTGCATTGGTATGGTTTCTCTCCAGAA38870.1737025044084775No Hit
GTGGCTGGCGAGGTTGCCCTTGTAGCGGAAGGAGGCCTGGCAGCGGTCAC38170.17057433993495205No Hit
GGATGGGCTGGTGCCTATTGTGACGTGCCCAATGTCTCTTGTGACATAGC37250.16646303805546145No Hit
GTGGTGTCGGAAACATACGTGTGAGGAGATTCTAGGGAATCAACAGGGGA36520.16320080939021347No Hit
GGACAGTCAATACTGCAGTGAATGACCTGAGCCCAAGGGAGCAAAGCTTA35370.15806168204085025No Hit
GAGAAGGTCCAACTGTCTGAGAGTTCAGTAACTTTATCCCCTGTTGATTC34930.15609540722891996No Hit
CAGCTTTCTATCACATAGTGAAGGACGACGCATTGTCGTTATCTGCGGTC34800.1555144623981224No Hit
GGTCAGGTTACGCAGCAAGGCCATGCCAGTTTCTGTCAGCCACACACCTT34240.15301193081930203No Hit
TTGTAAGTTCGAAATGGGCACAGTTTGTACTCCCCCTGAGGGTGGCATTG34220.15292255469148702No Hit
TTTGTCCTCTTTGTCAAATGTCACGACTTCGGCCACACAGTTAGGGGCAC33760.15086690375174172No Hit
CTCTGAAATCACCTGTTAACGATGTTATTGAGCCGGCTGGCTTGTGGGAT33350.14903469313153395No Hit
CCTCTTATGCAGAGCCCCAGTACTTTCGCTTCGAAGGTGTGTGGCTGACA33020.14755998702258624No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT32830.1467109138083436No Hit
CTGTAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAG32690.14608528091363854No Hit
CGTTCAGGTGGCCCACCTCCGTGCCCATGTGCTTATCCACACTGGTGAGA32610.1457277764023785No Hit
GAACTTGTATTTCTTCCAGTTGCAGGCTTTGGGGTCAGTGGGGCTCTTGG32080.14335930901528063No Hit
CACAGAGCATGTGGAGGCCTTTATCACCATCCAGGTGGCCCAGATGGTGA31760.14192929097024043No Hit
GATGTTCATGATATTTTGTTACACGTTCATTGTCAAAACCTTGGTGCAAG31440.14049927292520023No Hit
GACCTCCAGTCCCCAACCAAGCTGAGTGCCAGCGGGGAGGACTCCACCAT31310.13991832809440263No Hit
GGGGCTGCTGAACTTGTACTTGTACCTGCTGTGGTGGGGGTGCTGGTGAA31300.13987364003049513No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC31180.13933738326360506No Hit
CTCCTGTGCCGGGAGGTACTCAGAAAACATTTCTCGGCAGACCAGTGAGA31130.13911394294406754No Hit
ACACGGATGGCTTTGTGCCTTTTAGAATTCTGAGCTTGCACCAAGGTTTT31010.13857768617717744No Hit
CTTTCAGCCAATAGCAATCCTTGTGAGCATGCAGGAAAATGTGTGAACAC30990.13848831004936243No Hit
TGCTCTGCAGGTAATCATCAGAGACAACCACCACCATCCGGCGGCACCTA30810.1376839248990273No Hit
AGAGAGGTATAGAGGGGATAACAGATGCCGAGTACCTGGCATGGAGGGGT30740.13737110845167477No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA30600.13674547555696967No Hit
CACACCCCAATGGCGGGATAGCAGCCCCTGTGTGTGAGCACCCCTCCATG30100.13451107236159437No Hit
AACAGAAGCTCATGGCCAGCAGGGGGACCAGAAAGCCGATCACCATCTGG30010.1341088797864268No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA29960.13388543946688927No Hit
CCCTGACTCTGTCCCACTGCCCAGGTACATTAACCATTCCTGTGCCCCTA29860.13343855882781422No Hit
AATTTCGAATAGCCGGTCTAGTTTGTCCTCAGCCTGGGGAATCTCTTCAA29810.13321511850827666No Hit
CAATACCACAATAACCAGGGTACCTTCTGCCAGGTTCTCAGGTTGGTCAG29530.13196385271886651No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG29500.13182978852714397No Hit
AGGACATCTCTGATTGGAGGGGGGACTCTTGCTCATAGGAGATGATGGTC29330.13107009144071635No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT29280.13084665112117883No Hit
CCATAACAGCTTTTTTCTCTCCTTGAAGTATTTGTCTGAAAGTGGCCACC29260.13075727499336384No Hit
CCTGTGTAGTTCCGCCACTTGCCAATGCCTTTCCACTGGAAGCAGGAAGA28990.12955069726786117No Hit
TGTAAGCCAGCCAGCTCAACAAGGTCAAACTTATGCACCAGCCATTCACC28540.12753973439202337No Hit
CGAGACACAAAGCATGGATGAGATGTTGAGTACTGGCAGTCCTCTCCTGT28330.1266012850499657No Hit
GACAGAGAGAGTAGTTCTCAAACCACTCCGAGTTGGGATTCAGTTCTGCT28210.12606502828307564No Hit
ACCACCACACTCACCTTTCCACGGTCATCTTGATGGTAGCTGGGACATAA27760.12405406540723786No Hit
CTCACAACGAGGTCCTGCATAACCCTTCAGACACTCACAGTGGAAGGCGC27730.12392000121551534No Hit
ATGTTAGGTTGTCCAGTTAGCTGAACTGTCTGACCAGCTGGAAGAGCTGC27700.12378593702379283No Hit
GGACACCTATAATACTGAGCTCCTGAAATCAGATTCAGACAATAACAACA27700.12378593702379283No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG27520.1229815518734577No Hit
GAAGGTGCCGGAAACGGGTGCCACAGATTTCACAGGGATAGGGCTTCTCA27480.12280279961782768No Hit
CACCCAGCCCAAACTACCAAAAATATACATATGGCCAATCCAGCATGAGC27430.12257935929829014No Hit
ACAAAGTCCATAATGTCTGAAGGCAGGATATTCCCACAGTCATCACTGTT27320.12208779059530758No Hit
GAACCAATCAAGCATTGCCCAAGATCGAGGTGAGAGCCTGGGTGTTGGGG27270.12186435027577004No Hit
CTTTTTCTTCCCTCCTCTCCCCCAACTGCAGGATGCCTTTGTGGAACTGT27260.12181966221186255No Hit
GTACTTGATGGGGATCAGTCGCTTCTGATGGGCACCTGCAAGCCAAGGGG27150.12132809350887998No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT27120.12119402931715743No Hit
GACTCATACCTCCATAACTCGACTTATTGTCCTGAAGTGTTTGTATAGGC27070.12097058899761992No Hit
TCCAGTGTGATGATGGTGAGGATGGGCCTCCGGTTCATGCCGCCCATGCA26990.12061308448635986No Hit
GGAGAAGCAACGGCAGGTAAGTTGACACCCTGGAGCTAAGCCCCCTATAT26890.1201662038472848No Hit
CTCCCCTACTCGGTGCTGCAGCCTCCGCAAGCCTGTCTTAAACTTGAGTT26890.1201662038472848No Hit
CGGTGCGCAGCCGCCGGTACTGAGATGACTTGGAGTGCACAAACTGCTTG26540.11860212161052207No Hit
CTAACCTTCATGAAATGCAGCCTTTGGCACATGGGGCCAGCACTGTGCTT26430.11811055290753951No Hit
AAGAATGCCTGCATCCTCCAGGCTTCTGGCTCCCCTCCAGCCAAGAGCCC26360.11779773646018694No Hit
GGTGTGGAATATCTGCCTTGGTCTTTGAGAATCCTTGATAAAATTCTTTC26170.11694866324594433No Hit
CTCCTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTA26160.11690397518203682No Hit
CCTCACACAACACACCAGTGAAACCTTTGGAAAGAATTTTATCAAGGATT26140.11681459905422181No Hit
CTACTCCAATCATAGGGCTGCCCCAGAGACAGGAGCGATATAGGGGGCTT26130.11676991099031431No Hit
CACAAAAGCCAGCTGCGACTTCACTTGCGCCAGAAGCATGGCGCCATCAC26060.11645709454296176No Hit
GGAAGGTGGCATGGTGCCCATGGGCTTCTGTGATAGCACTGGCTGTGAGC25980.11609959003170171No Hit
CTGCCGAAGCCTTGGCGTTCTCAGGTGAGTGCAGGGTTTGCTCCTGGAGG25980.11609959003170171No Hit
CAAGGGATTTCCATTTCTCTTTCGATGCCGACATACTTCAGGGCATCAGC25860.11556333326481164No Hit
GGGACTGCTGAGACTGGGGCTGAGGATACGCAGCCTGCTGGGAGAGCGTC25790.11525051681745908No Hit
GTGACCAGGGGTGCAACAGTGAGGAGTGTGGTTGGGATGGGCTGGACTGT25740.11502707649792154No Hit
GGACTTTCAGGTGAATCCCTGACTCCAGAACGCACTCAGCTCCTCTATCT25710.11489301230619903No Hit
AGAGCAAGGTCATGGCAACAATCAGGATTTAACTAGGAACAGTAGCACCC25710.11489301230619903No Hit
CGTGCTAATTGCATTAACCTGCCTGGATGGTACCACTGTGAGTGCAGAGA25650.114624883922754No Hit
AGAAGGAGGAGCTGCTGTTGGTATAGTGCTGAGGGTTCCAATGTTACCAG25610.11444613166712399No Hit
GACAAAGCAAGGTCAAGTATCCCCTCTGGTCCCTGCTGGTACCTCCAGGA25360.11332893006943631No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC25340.11323955394162132No Hit
CACAGAAAAATCTGTAGCCTCCTCTGAAAGTGACCCAGCACTGCTGAGCC25320.11315017781380629No Hit
CACATGTGCCCGGCGGGGTTGTGGGCTTCGCTCAGGCCTGGGTGGGGAGC25280.11297142555817626No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG25210.11265860911082372No Hit
GTCCAATCAAGGGCATATGCTAAGTGGGCAGCATGGAGGGCAGGCAGGCT25210.11265860911082372No Hit
ATGGAGGTCCTTATGACAGAGTGAGGTAAGCATGACCCCAGCTCCTGTCC25190.11256923298300871No Hit
GAAACGGCGAGAAGAGGAAAAGGTGTCAGGTGTGGTTGGAGACCCCCAGA25180.1125245449191012No Hit
TGTCATCCACGTCCTGGAGCCAGGAGATGAACTGGCGGGCGTTGAAACGG25060.11198828815221111No Hit
CAGAGACAGCCAGGAGAAATCAAACAGAGGCCGCATGCTGGGGCCGTACA24860.11109452687406099No Hit
CCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGGTGAGTAGCTGC24760.11064764623498592No Hit
GTGGGCCCTGTGCCAGCCTGGTGACCTGCCCTATCTGTCATGCTCCTTAC24700.11037951785154089No Hit
CCCTCAGCAAAAGGCCAAAGATAACCGGGCAGCTCACCGCATCAACAAGG24670.11024545365981837No Hit
GTGGTCGTGGTCTTCATAGTCTTCCAGCTGCCCTACAATGGGGTGGTCCT24640.11011138946809586No Hit
GAACCAAGGAACCTGCTTTGATGACATAAGTGGCTACACTTGCCACTGTG24620.11002201334028083No Hit
CGAAAACCTCGTGGGAGACCTAGAAGTGGCTCTGACCGAAATTCAGCTAT24610.10997732527637334No Hit
GACCCAGTCCCGGGTGCTCAGTTCCCCCCAGCTGGCACAGCAGGGTCAGG24380.10894949980650068No Hit
ACTTGAAAAGCAGATTCCTTGGTAAGGATGCCTCCGCCTGAACCGAAGTA24350.10881543561477816No Hit
AGAGGACTGCTACACCCCAGCCCAGCCCCACTCACCTTCTGCACCTTGTT24280.10850261916742561No Hit
AATGATGTTGGTGATGAGAGAAGGTTGAGATTATCCAAAAGATTTTCCAT24240.1083238669117956No Hit
GACATGCTCCACTTTCTAAATCCTGAGGAGCTGCGGGTGATTGAAGAGAT24190.10810042659225806No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG24180.10805573852835056No Hit
GCCCTCGAGGCCACGGGATTCTTCATCTGCAATATCTGGACCATCATCTC24120.10778761014490551No Hit
CTAAGCAATTAGTACAAACACCACAGTCCTCAGGCACCTGGCAGCCGGGA24100.10769823401709051No Hit
AGGGCACTCAAATGTCTGCCCTAGCTCCCTGCCCCTCCCCAACACCCAGG24030.10738541756973796No Hit
GTGGACAGAGGCTGAGTTTGAAGAGAAGTGTACATACATTGTGAACGACC23980.10716197725020042No Hit
CCTCCTTAGCCCACTGATGAACGATGGCTCCAACTCTGGTAACATTGGAA23910.10684916080284788No Hit
GCTCTTAGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGGAAATT23890.10675978467503287No Hit
GTCAGTCACGACCCACCTTGTCCTGGATATTGGGATTTGAGCCGAAGCTG23750.10613415178032778No Hit
GGCTGAGCTGTGGTGGTGCTAAGGAATCCAAGCTGAAAGAAACAGAAGGG23700.10591071146079024No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT23600.10546383082171518No Hit
GAACAAGAGCAATGACTTTGAGGAACTGGTTGAGCGGAGCCAGCTGGACA23600.10546383082171518No Hit
TGATGTTGTTCTCACAATGGACTCCATCAAATCCTGCCTGGCACTTGCAA23540.10519570243827014No Hit
GGGCACATACTTCTGGTTCTGGATGGGAACAGTTGGGGAAACAGGTACCT23500.10501695018264011No Hit
GCCCACTCCCTTCCCTTAGGCGTGCCGGGTGTGCCGGGCCTGTGGGGCGG23500.10501695018264011No Hit
CACAGTAGGTGATCCTTTACTCTCCTCTGGACTTCGAAGCATTGGCTCCA23480.10492757405482511No Hit
CAGTGCATGGATAGGATTGGAGGCTACAGTTGTCGCTGCTTGCCTGGCTT23470.1048828859909176No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG23470.1048828859909176No Hit
GAACAGCACCAGCATGTCTAAGGCATATCAGAGCACCTTCACAGGCGAGA23350.10434662922402752No Hit
CTGATCTGTATCCTAAATCCTCATAATGGGACCAGAGGATCCCTGTCAAC23240.10385506052104496No Hit
CCAGCACTGCCACTGCCTGGAGACACAATGTGGTGGTGGGATAGCAACTG23180.10358693213759992No Hit
GACCTTCACACGATTCTCCACTTGGTGAAAACATCCCATTGTCATGGTAG23150.10345286794587741No Hit
CCCGCAGCAGGGCGTGGAGGGGGCTGGGGAGGAAATCCTTGGGAGCCCCG23130.10336349181806238No Hit
ATGTGCCCTGTGCACCCTCACCTTGTGTCAATGGAGGCACCTGTCGGCAG22820.10197816183692968No Hit
GTCCCCAGATGCCCTGCCTGAATGGAGGGACTTGTGCTGTGGCCAGTAAC22790.10184409764520716No Hit
GAGAGGACTGGAGCTGTGGTGGTTGAGACTGTGGCTGCTGCTGATACGAA22780.10179940958129965No Hit
ACCCAGTCTGTCTGAGATAAGCAATCCCGAAAACATGGAAAATCTTTTGG22690.1013972170061321No Hit
GAAGGAAGAGCGGGACAGCTACAATGACGAGCTGGTCAAGGTGAAGGACG22610.10103971249487205No Hit
CAGTAAGACCCGAGACTCCATGGTGCGGATCAGCTCAGTCCATCGTCTCC22550.100771584111427No Hit
CAACACTCCATGCTTCAGCAGGCTTTGGGGAGCTCCGGAGGCAGGTCAGT22540.1007268960475195No Hit
GCCATCTTGTCCTCAGCATAACATATCATAGCTTTTTATTTATCTCTCTC22500.10054814379188946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)