FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237734
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN125720.5618183394451709No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG104830.46846497394238995No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG75810.33878021248280626No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT73070.3265356829721495No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG59450.26567053993012574No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG58030.25932483485525987No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG57830.2584310735771097No Hit
AGAAAGGGGTTTGTGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCAC48270.21570928448153356No Hit
GTTTAGGTTTCAAAGGTGTGCATTGTGAATTAGAAATAAATGAATGTCAG47350.21159798260204296No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT46170.2063247910609572No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG45560.2035988191625993No Hit
GTCCACAGTGGGGGTCCGGTGCGGCGTTGTGCTCTCTGTAACTGCGGGGA45430.2030178743318017No Hit
GTGATGTCTCGATTGGCAAAATGGTCTAACAGGATCTTGGCTGCTTCATA45230.2021241130536516No Hit
CTGTAGGAAGAGACACCTCTGTTTCTTGCTGCCCGGGAGGGGAGCTATGA45150.20176660854239153No Hit
CGATACACTTTGCCCCATTCAGACACGGAGTACTGGAACAGTCATCAATA44620.1993981411552937No Hit
GTTTTCTCTGCAGTCTTTAAACCAGAGGAGTTACGCCAGGCCCTCATGCC43920.19626997668176827No Hit
CAAAGCGCCGTAGCTCCCGCTGCCCGTGTAGACTGGGCTCCCCGCAGTTA43880.19609122442613824No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA43600.19483995863672804No Hit
AGATTTTCGCTTCCCTTGCATAGGAGCAGTGGAAGCAGGCAAAAATGGTG43510.1944377660615605No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC43030.19229273899400018No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC42370.18934332677610477No Hit
GACCACACACCTTGGCTTATGCCTCCAACAATCCCCTTAGCATCACCATT42050.18791330873106454No Hit
AGCAGCACCAGCAATCTCAAGACCCACCTGCGACTCCATTCTGGAGAGAA41780.18670673100556187No Hit
GGAGAGGAACGACTGGCATCCCATAATTTATTTCGGGAGGAGGAACAGTG41500.1854554652161517No Hit
CATCAACACCATCCAACTGTGAGATTTTTGGAAGATCACACTGTTCCTCC40840.18250605299825626No Hit
CTGGGTCCTGTCGATACAGTGCTTCTAGGGTTGGCATGAGGGCCTGGCGT40570.18129947527275359No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT40470.18085259463367853No Hit
TTTGAGGTTTCACTGGGCCAGTTTGCCAGATTGATATTGATGACTGTTCC40190.17960132884426835No Hit
GGGTGAACTTGGCAGGGCACACCTTGCATTGGTATGGTTTCTCTCCAGAA39680.17732223758498553No Hit
ATCGCCTCTATGTGTCTGTGAAGGAGATTGGTGGATTGACTCAGGTGAGT39310.17566877922040777No Hit
ATCCACACACTGCCCATTGTTCACACAAGGGTTGCTCTGACATTCATTTA39300.17562409115650027No Hit
CAAGAGGCACACGCTGCAGACCCACAGTGACAAACCCTACAAGTGTGACC39150.1749537701978877No Hit
CAAATGTTATCTGTGAAAAGGCAGCAACAGCAGCAACATTCACCAGCACC37730.16860806512302176No Hit
GTGGCTGGCGAGGTTGCCCTTGTAGCGGAAGGAGGCCTGGCAGCGGTCAC37120.16588209322466388No Hit
GTGGTGTCGGAAACATACGTGTGAGGAGATTCTAGGGAATCAACAGGGGA36580.16346893777365853No Hit
GGATGGGCTGGTGCCTATTGTGACGTGCCCAATGTCTCTTGTGACATAGC36360.1624858003676934No Hit
GGACAGTCAATACTGCAGTGAATGACCTGAGCCCAAGGGAGCAAAGCTTA35770.1598492045971505No Hit
CCTCTTATGCAGAGCCCCAGTACTTTCGCTTCGAAGGTGTGTGGCTGACA35250.15752542527396018No Hit
TTGTAAGTTCGAAATGGGCACAGTTTGTACTCCCCCTGAGGGTGGCATTG34730.15520164595076985No Hit
GAGAAGGTCCAACTGTCTGAGAGTTCAGTAACTTTATCCCCTGTTGATTC34720.15515695788686235No Hit
CTCCTGTGCCGGGAGGTACTCAGAAAACATTTCTCGGCAGACCAGTGAGA33670.15046471117657417No Hit
CTCTGAAATCACCTGTTAACGATGTTATTGAGCCGGCTGGCTTGTGGGAT33430.14939219764279402No Hit
ACACGGATGGCTTTGTGCCTTTTAGAATTCTGAGCTTGCACCAAGGTTTT33290.14876656474808891No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC32740.14630872123317606No Hit
CAGCTTTCTATCACATAGTGAAGGACGACGCATTGTCGTTATCTGCGGTC32650.14590652865800852No Hit
CCATAACAGCTTTTTTCTCTCCTTGAAGTATTTGTCTGAAAGTGGCCACC32380.14469995093250582No Hit
TTTGTCCTCTTTGTCAAATGTCACGACTTCGGCCACACAGTTAGGGGCAC32290.14429775835733827No Hit
GGTCAGGTTACGCAGCAAGGCCATGCCAGTTTCTGTCAGCCACACACCTT32040.1431805567596506No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT31700.1416611625867954No Hit
CGTTCAGGTGGCCCACCTCCGTGCCCATGTGCTTATCCACACTGGTGAGA31690.1416164745228879No Hit
AGAGAGGTATAGAGGGGATAACAGATGCCGAGTACCTGGCATGGAGGGGT31650.14143772226725787No Hit
CACAGAGCATGTGGAGGCCTTTATCACCATCCAGGTGGCCCAGATGGTGA31520.14085677743646027No Hit
GAACTTGTATTTCTTCCAGTTGCAGGCTTTGGGGTCAGTGGGGCTCTTGG31370.1401864564778477No Hit
TGCTCTGCAGGTAATCATCAGAGACAACCACCACCATCCGGCGGCACCTA31140.13915863100797504No Hit
GGGGCTGCTGAACTTGTACTTGTACCTGCTGTGGTGGGGGTGCTGGTGAA31090.1389351906884375No Hit
CCCTGACTCTGTCCCACTGCCCAGGTACATTAACCATTCCTGTGCCCCTA31020.13862237424108495No Hit
GACCTCCAGTCCCCAACCAAGCTGAGTGCCAGCGGGGAGGACTCCACCAT30960.13835424585763992No Hit
CTTTCAGCCAATAGCAATCCTTGTGAGCATGCAGGAAAATGTGTGAACAC30830.13777330102684235No Hit
CTGTAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAG30810.1376839248990273No Hit
AATTTCGAATAGCCGGTCTAGTTTGTCCTCAGCCTGGGGAATCTCTTCAA30460.1361198426622646No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA30450.1360751545983571No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG30410.13589640234272707No Hit
CACACCCCAATGGCGGGATAGCAGCCCCTGTGTGTGAGCACCCCTCCATG30380.13576233815100455No Hit
AACAGAAGCTCATGGCCAGCAGGGGGACCAGAAAGCCGATCACCATCTGG30220.13504732912848444No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG30200.13495795300066943No Hit
GATGTTCATGATATTTTGTTACACGTTCATTGTCAAAACCTTGGTGCAAG29860.13343855882781422No Hit
GGACACCTATAATACTGAGCTCCTGAAATCAGATTCAGACAATAACAACA28710.12829943147845096No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA28620.12789723890328342No Hit
CGAGACACAAAGCATGGATGAGATGTTGAGTACTGGCAGTCCTCTCCTGT28590.1277631747115609No Hit
CTCCCCTACTCGGTGCTGCAGCCTCCGCAAGCCTGTCTTAAACTTGAGTT28420.12700347762513328No Hit
CAATACCACAATAACCAGGGTACCTTCTGCCAGGTTCTCAGGTTGGTCAG28420.12700347762513328No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT28350.12669066117778074No Hit
ATGTTAGGTTGTCCAGTTAGCTGAACTGTCTGACCAGCTGGAAGAGCTGC28330.1266012850499657No Hit
GACAGAGAGAGTAGTTCTCAAACCACTCCGAGTTGGGATTCAGTTCTGCT28130.1257075237718156No Hit
CACCCAGCCCAAACTACCAAAAATATACATATGGCCAATCCAGCATGAGC28120.1256628357079081No Hit
AGGACATCTCTGATTGGAGGGGGGACTCTTGCTCATAGGAGATGATGGTC28090.12552877151618555No Hit
CTCACAACGAGGTCCTGCATAACCCTTCAGACACTCACAGTGGAAGGCGC27960.124947826685388No Hit
TCCAGTGTGATGATGGTGAGGATGGGCCTCCGGTTCATGCCGCCCATGCA27730.12392000121551534No Hit
CCTGTGTAGTTCCGCCACTTGCCAATGCCTTTCCACTGGAAGCAGGAAGA27580.12324968025690274No Hit
CTACTCCAATCATAGGGCTGCCCCAGAGACAGGAGCGATATAGGGGGCTT27450.12266873542610517No Hit
GAAGGTGCCGGAAACGGGTGCCACAGATTTCACAGGGATAGGGCTTCTCA27330.12213247865921507No Hit
GACTCATACCTCCATAACTCGACTTATTGTCCTGAAGTGTTTGTATAGGC27190.12150684576450999No Hit
GTGACCAGGGGTGCAACAGTGAGGAGTGTGGTTGGGATGGGCTGGACTGT27110.12114934125324994No Hit
AAGAATGCCTGCATCCTCCAGGCTTCTGGCTCCCCTCCAGCCAAGAGCCC27090.12105996512543492No Hit
GAACCAATCAAGCATTGCCCAAGATCGAGGTGAGAGCCTGGGTGTTGGGG26930.12034495610291482No Hit
TGTAAGCCAGCCAGCTCAACAAGGTCAAACTTATGCACCAGCCATTCACC26820.11985338739993225No Hit
ACAAAGTCCATAATGTCTGAAGGCAGGATATTCCCACAGTCATCACTGTT26770.1196299470803947No Hit
CTGCCGAAGCCTTGGCGTTCTCAGGTGAGTGCAGGGTTTGCTCCTGGAGG26770.1196299470803947No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT26620.11895962612178212No Hit
GTACTTGATGGGGATCAGTCGCTTCTGATGGGCACCTGCAAGCCAAGGGG26550.11864680967442957No Hit
GGTGTGGAATATCTGCCTTGGTCTTTGAGAATCCTTGATAAAATTCTTTC26540.11860212161052207No Hit
CTTTTTCTTCCCTCCTCTCCCCCAACTGCAGGATGCCTTTGTGGAACTGT26380.11788711258800197No Hit
CCTCACACAACACACCAGTGAAACCTTTGGAAAGAATTTTATCAAGGATT26330.11766367226846443No Hit
GGAGAAGCAACGGCAGGTAAGTTGACACCCTGGAGCTAAGCCCCCTATAT26320.11761898420455694No Hit
CTCCTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTA26260.1173508558211119No Hit
ACCACCACACTCACCTTTCCACGGTCATCTTGATGGTAGCTGGGACATAA26230.11721679162938936No Hit
GCCCTCGAGGCCACGGGATTCTTCATCTGCAATATCTGGACCATCATCTC26190.11703803937375935No Hit
CCCTCAGCAAAAGGCCAAAGATAACCGGGCAGCTCACCGCATCAACAAGG25970.11605490196779421No Hit
AGAAGGAGGAGCTGCTGTTGGTATAGTGCTGAGGGTTCCAATGTTACCAG25950.11596552583997918No Hit
AGAGCAAGGTCATGGCAACAATCAGGATTTAACTAGGAACAGTAGCACCC25860.11556333326481164No Hit
GGACTTTCAGGTGAATCCCTGACTCCAGAACGCACTCAGCTCCTCTATCT25830.11542926907308912No Hit
CTAACCTTCATGAAATGCAGCCTTTGGCACATGGGGCCAGCACTGTGCTT25740.11502707649792154No Hit
CACATGTGCCCGGCGGGGTTGTGGGCTTCGCTCAGGCCTGGGTGGGGAGC25730.11498238843401405No Hit
CAAGGGATTTCCATTTCTCTTTCGATGCCGACATACTTCAGGGCATCAGC25260.11288204943036126No Hit
CGGTGCGCAGCCGCCGGTACTGAGATGACTTGGAGTGCACAAACTGCTTG25240.11279267330254623No Hit
CAGAGACAGCCAGGAGAAATCAAACAGAGGCCGCATGCTGGGGCCGTACA25170.11247985685519368No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG25070.11203297621611863No Hit
GTCCAATCAAGGGCATATGCTAAGTGGGCAGCATGGAGGGCAGGCAGGCT25070.11203297621611863No Hit
AATGATGTTGGTGATGAGAGAAGGTTGAGATTATCCAAAAGATTTTCCAT25000.11172015976876608No Hit
GCTCTTAGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGGAAATT24950.11149671944922855No Hit
GACAAAGCAAGGTCAAGTATCCCCTCTGGTCCCTGCTGGTACCTCCAGGA24940.11145203138532105No Hit
GGAAGGTGGCATGGTGCCCATGGGCTTCTGTGATAGCACTGGCTGTGAGC24910.11131796719359853No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG24900.11127327912969101No Hit
ACTTGAAAAGCAGATTCCTTGGTAAGGATGCCTCCGCCTGAACCGAAGTA24770.11069233429889343No Hit
CGTGCTAATTGCATTAACCTGCCTGGATGGTACCACTGTGAGTGCAGAGA24750.11060295817107843No Hit
GTCCCCAGATGCCCTGCCTGAATGGAGGGACTTGTGCTGTGGCCAGTAAC24620.11002201334028083No Hit
CAACACTCCATGCTTCAGCAGGCTTTGGGGAGCTCCGGAGGCAGGTCAGT24590.10988794914855832No Hit
CCAGCACTGCCACTGCCTGGAGACACAATGTGGTGGTGGGATAGCAACTG24570.1097985730207433No Hit
GACCCAGTCCCGGGTGCTCAGTTCCCCCCAGCTGGCACAGCAGGGTCAGG24560.1097538849568358No Hit
GTGGGCCCTGTGCCAGCCTGGTGACCTGCCCTATCTGTCATGCTCCTTAC24550.10970919689292828No Hit
GAACCAAGGAACCTGCTTTGATGACATAAGTGGCTACACTTGCCACTGTG24490.10944106850948325No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC24480.10939638044557576No Hit
ATGGAGGTCCTTATGACAGAGTGAGGTAAGCATGACCCCAGCTCCTGTCC24450.10926231625385324No Hit
CCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGGTGAGTAGCTGC24430.10917294012603822No Hit
GAACAGCACCAGCATGTCTAAGGCATATCAGAGCACCTTCACAGGCGAGA24390.10899418787040818No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG24250.1083685549757031No Hit
GAAACGGCGAGAAGAGGAAAAGGTGTCAGGTGTGGTTGGAGACCCCCAGA24200.10814511465616555No Hit
CCCGCAGCAGGGCGTGGAGGGGGCTGGGGAGGAAATCCTTGGGAGCCCCG24160.10796636240053555No Hit
CACAGAAAAATCTGTAGCCTCCTCTGAAAGTGACCCAGCACTGCTGAGCC24090.107653545953183No Hit
CTAAGCAATTAGTACAAACACCACAGTCCTCAGGCACCTGGCAGCCGGGA24060.10751948176146048No Hit
GACCTTCACACGATTCTCCACTTGGTGAAAACATCCCATTGTCATGGTAG24040.10743010563364545No Hit
CACAAAAGCCAGCTGCGACTTCACTTGCGCCAGAAGCATGGCGCCATCAC24030.10738541756973796No Hit
CAGTGCATGGATAGGATTGGAGGCTACAGTTGTCGCTGCTTGCCTGGCTT24030.10738541756973796No Hit
GTGGACAGAGGCTGAGTTTGAAGAGAAGTGTACATACATTGTGAACGACC24010.10729604144192294No Hit
GACATGCTCCACTTTCTAAATCCTGAGGAGCTGCGGGTGATTGAAGAGAT24000.10725135337801545No Hit
CCTCCTTAGCCCACTGATGAACGATGGCTCCAACTCTGGTAACATTGGAA23900.10680447273894036No Hit
GGGCACATACTTCTGGTTCTGGATGGGAACAGTTGGGGAAACAGGTACCT23900.10680447273894036No Hit
AGAGGACTGCTACACCCCAGCCCAGCCCCACTCACCTTCTGCACCTTGTT23840.10653634435549533No Hit
GCCCACTCCCTTCCCTTAGGCGTGCCGGGTGTGCCGGGCCTGTGGGGCGG23770.10622352790814278No Hit
CGAAAACCTCGTGGGAGACCTAGAAGTGGCTCTGACCGAAATTCAGCTAT23670.10577664726906773No Hit
GTCAGTCACGACCCACCTTGTCCTGGATATTGGGATTTGAGCCGAAGCTG23630.10559789501343769No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT23600.10546383082171518No Hit
GGGACTGCTGAGACTGGGGCTGAGGATACGCAGCCTGCTGGGAGAGCGTC23340.10430194116012002No Hit
GAAGGAAGAGCGGGACAGCTACAATGACGAGCTGGTCAAGGTGAAGGACG23320.104212565032305No Hit
CTGATCTGTATCCTAAATCCTCATAATGGGACCAGAGGATCCCTGTCAAC23290.10407850084058248No Hit
AGGGCACTCAAATGTCTGCCCTAGCTCCCTGCCCCTCCCCAACACCCAGG23230.10381037245713745No Hit
TGTCATCCACGTCCTGGAGCCAGGAGATGAACTGGCGGGCGTTGAAACGG23180.10358693213759992No Hit
CAGGAGAGCCCCTACCTTTTTTGGCTCCCTCAGGAACAATCCTGTACACT23110.10327411569024736No Hit
TCGATTTTTATTTTTATTTTTAAAGAGGGAGACGATGGACTGAGCTGATC23090.10318473956243235No Hit
GTGGTCGTGGTCTTCATAGTCTTCCAGCTGCCCTACAATGGGGTGGTCCT23000.1027825469872648No Hit
TCTTCTGCAGTCTGGAGAGATGGCCCTCCACGTGGCAGCTCGCTATGGCC22930.10246973053991223No Hit
ATGTGCCCTGTGCACCCTCACCTTGTGTCAATGGAGGCACCTGTCGGCAG22830.1020228499008372No Hit
CACAGTAGGTGATCCTTTACTCTCCTCTGGACTTCGAAGCATTGGCTCCA22820.10197816183692968No Hit
GAACAAGAGCAATGACTTTGAGGAACTGGTTGAGCGGAGCCAGCTGGACA22650.10121846475050207No Hit
GGCTGAGCTGTGGTGGTGCTAAGGAATCCAAGCTGAAAGAAACAGAAGGG22480.10045876766407447No Hit
ACCCAGTCTGTCTGAGATAAGCAATCCCGAAAACATGGAAAATCTTTTGG22410.10014595121672192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)