FastQCFastQC Report
Wed 8 May 2024
EGAF00004965474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944575
Total Bases65.9 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18861519.9682396845142No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC378784.01005743323717No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG369073.9072598787814625No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGC153971.6300452584495675No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG118691.2565439483365535No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG118061.2498742820845354No Hit
CAACAGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACA78130.8271444829685307No Hit
CGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACAGCAA65200.6902575232247307No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAACAACAACAGC58710.6215493740571156No Hit
GCTGTTGTTGTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG58290.6171029298891035No Hit
GGGCAAGCTGGAGTACCTGTACCTGCAGGCCAACCTCATCGAGGTGGTC36180.38302940475875397No Hit
GACCACCTCGATGAGGTTGGCCTGCAGGTACAGGTACTCCAGCTTGCCC35770.37868882830902784No Hit
CAGGTGGTGGTGACAGGCGTGATGCCTCAGGTGGTGGGG22740.24074319138236772No Hit
CCCCACCACCTGAGGCATCACGCCTGTCACCACCACCTG22150.23449699600349364No Hit
GCAGCCCCAGCATCAGGCCCTTCCCACAGGGGAGCCCTGGCTCC15800.1672709948918826No Hit
GGAGCCAGGGCTCCCCTGTGGGAAGGGCCTGATGCTGGGGCTGC15510.16420083106158853No Hit
GCCGAGTCCCCACTGGCCACCATGTGGGGCACGCAGGAGC15400.16303628616044252No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCC14230.15064976312098033No Hit
CCAGGACCCTGCTGCTGTTGCTGCTGGATTGCCACCTGTCCTAGAAGGTG14160.14990868909297833No Hit
GCTCCTGCGTGCCCCACATGGTGGCCAGTGGGGACTCGGC13200.13974538813752216No Hit
GGCTCTCCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG13030.13794563692666012No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG13000.13762803377180213No Hit
CCCCAGGCACTGATACTCACCTGACTGAGGTTGAAGGTCATGATGCTGTT11850.12545324616891196No Hit
AGATACCATCAACATCCTGCTGTATGATGACAACAGCATCATGACCTTCA11740.12428870126776594No Hit
ACTGACCTTGTGGGCCATACTGACCCCCCTGGGGACCATAGCTCCCCATG10550.11169044279173174No Hit
TGTTGCCATGACCTTGCTCTACTGAACCACAAGGAGGGCTAGAGATGTGG10350.1095730884260117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)