FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004965475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944575
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18597219.688431305084297No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG369283.9094831008654687No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC365633.870841383691078No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG116341.2316650345393432No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG114121.2081624010798506No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGCTGTTG69150.7320752719477014No Hit
CAACAGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC68700.7273112246248312No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGCG58650.6209141677473997No Hit
CGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC58080.6148797078050976No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAACAACAACAGC57760.6114919408199455No Hit
GCTGTTGTTGTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG56800.6013286398644894No Hit
GACCACCTCGATGAGGTTGGCCTGCAGGTACAGGTACTCCAGCTTGCCC35360.3743482518593018No Hit
GGGCAAGCTGGAGTACCTGTACCTGCAGGCCAACCTCATCGAGGTGGTC35050.37106635259243576No Hit
CCCCACCACCTGAGGCATCACGCCTGTCACCACCACCTG22430.23746129211550168No Hit
CAGGTGGTGGTGACAGGCGTGATGCCTCAGGTGGTGGGG21250.2249689013577535No Hit
GGAGCCAGGGCTCCCCTGTGGGAAGGGCCTGATGCTGGGGCTGC15870.1680120689198846No Hit
GCAGCCCCAGCATCAGGCCCTTCCCACAGGGGAGCCCTGGCTCC15190.1608130640764365No Hit
GCTCCTGCGTGCCCCACATGGTGGCCAGTGGGGACTCGGC14730.15594314903528042No Hit
GGCTCTCCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG13760.14567398036153825No Hit
GCCGAGTCCCCACTGGCCACCATGTGGGGCACGCAGGAGC13070.13836910779980416No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCC12930.1368869597438001No Hit
AGATACCATCAACATCCTGCTGTATGATGACAACAGCATCATGACCTTCA11120.11772490273403383No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGC11060.11708969642431782No Hit
CCCCAGGCACTGATACTCACCTGACTGAGGTTGAAGGTCATGATGCTGTT10550.11169044279173174No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG10110.10703226318714766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)