FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004965477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294717
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN110540.853777311953114No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG33920.26198775485299103No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG25400.19618186831562417No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG24270.1874540922842598No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC24240.18722238141617048No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG22320.172392885858454No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT19800.15292917293895114No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT17390.13431506653577577No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG15330.11840425359364247No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA15110.11670504056098746No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA14390.11114397972684378No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT14300.11044884712257581No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT14210.10975371451830787No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC14150.10929029278212922No Hit
AGATACCATCAACATCCTGCTGTATGATGACAACAGCATCATGACCTTCA14090.10882687104595058No Hit
CCCACAAGGCGTCCTCTTCATCCTTCAGCGTCTCCATGTAGTCATCCATC14050.10851792322183149No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC13950.10774555366153377No Hit
CGCCTTGTCTCCTACAGGGAAACCAATGAAAAGCGTGCTGGTGGTGGCTC13760.10627805149696805No Hit
CGAAGTCCGTGTAGACGGTGTCCTTGAGCAGCGCCCCCGACCCGAAGTCG13480.1041154167281344No Hit
GTAGCTGCCCTGGGTGTTATGGCAGAGACCATGGTTCATGCAGGGCTTGG13340.10303409934371759No Hit
CCTGGCTCCTCAGGCCCTTCATCCGGCTCCAGCTCTGGCCCATCGAGGCC13180.1017983080472412No Hit
TGGTGGATACAGATGCGAGGTGAGGACACTGAGCAAGCAGAGGTCATGGA13170.10172107109121142No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG13120.10133488631106258No Hit
CCAAAGGGAAGGATGGGCTACATCACCCCCTTGCTTCTGCAGTCCAAACA13010.10048527979473507No Hit
GGCAGAGAACCTCACCTGGAAAATGACAAGGAGAGCCACCACCAGCACGC13010.10048527979473507No Hit
CCCCAGGCACTGATACTCACCTGACTGAGGTTGAAGGTCATGATGCTGTT12980.10025356892664575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)