FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2575336
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN664722.5811000972300313No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG95770.3718738059810448No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC77130.29949490085953834No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG75670.2938257376901499No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG67090.26050969659881273No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG62440.24245380020315796No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT62240.2416772025087212No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA47460.1842866328898443No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA44780.17388022378439164No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG44460.17263766747329282No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG44420.17248234793440545No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT38700.150271653873514No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT38490.14945622629435537No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG35240.13683651375975794No Hit
CCCACAAGGCGTCCTCTTCATCCTTCAGCGTCTCCATGTAGTCATCCATC32110.12468275984182259No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG31260.12138221964046635No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT31020.12045030240714222No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC30040.11664497370440206No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT29410.11419869096692627No Hit
GGGGTTTATGTCTAAAAGGAGGAGGGCCAGAGATGGATGACTACATGGAG29080.1129173047711056No Hit
ACAGGACCGCCAAGAAAAGAAGTTCCCAAAACCACTCCTAGTGAGCCCAA28570.11093698065029185No Hit
GCATCTTCCGGCCACACTCCTTGCAATCAACGAAACTAGGAGGCAAAGAA27710.10759761056421376No Hit
GGTTCCGTGTGCTTTAACAGTGCGCCTTCTTTGCCTCCTAGTTTCGTTGA27280.1059279255211747No Hit
TGCCCTCCTCACTCACCTGATTCTGGTGGTGGAGGCTGCTTTTTCTTGGG26990.10480185886424141No Hit
CAGAGTGAAACACCCACTTACTTGCAGCACTGCTTCTTTATATTGCGACC26930.10456887955591038No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA26750.10386994163091728No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC26330.10223908647260009No Hit
CCTCGGTGTCCGAGGACCTGGGGCTGGTGCATTTTCGGTTGTTGCTGATC26200.10173429797121618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)