FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2575336
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN656082.5475510768303633No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG91350.35471099693399233No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG75450.2929714802262695No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC74350.28870019290686727No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG69910.2714597240903711No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG61890.24031815654345684No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT60210.233794735910188No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA49920.19383878453141648No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG43950.17065734335247906No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG42660.16564828822336192No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA41460.16098870205674135No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT41400.1607557227484103No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT38770.15054346306656685No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG35170.13656470456670508No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT30930.12010083344464569No Hit
CCCACAAGGCGTCCTCTTCATCCTTCAGCGTCTCCATGTAGTCATCCATC30860.1198290242515928No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT30460.11827582886271928No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC30390.1180040196696664No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG29880.11602369554885264No Hit
GGGGTTTATGTCTAAAAGGAGGAGGGCCAGAGATGGATGACTACATGGAG29860.11594603577940897No Hit
ACAGGACCGCCAAGAAAAGAAGTTCCCAAAACCACTCCTAGTGAGCCCAA29760.1155577369321906No Hit
GCATCTTCCGGCCACACTCCTTGCAATCAACGAAACTAGGAGGCAAAGAA27910.10837420825865053No Hit
GGTTCCGTGTGCTTTAACAGTGCGCCTTCTTTGCCTCCTAGTTTCGTTGA27110.10526781748090347No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC26250.10192844739482537No Hit
ACTAATTGCTTAGATAAGCCCAAGTTTGGTGGTCGCAATATAAAGAAGCA26240.10188961751010353No Hit
AGCACATTACTTGGAAACACAGACCTCAAATGACTTTTCCAGTGTGAAGT26240.10188961751010353No Hit
CCTGCTGCCAAGAGGGTCAAGTTGGACAGTGTCAGAGTCCTGAGACAGAT26190.10169546808649435No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA26150.10154014854760698No Hit
CCTCGGTGTCCGAGGACCTGGGGCTGGTGCATTTTCGGTTGTTGCTGATC25890.10053057154483919No Hit
AGATACCATCAACATCCTGCTGTATGATGACAACAGCATCATGACCTTCA25850.10037525200595183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)