FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251334
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN364921.6209056497170125No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG75550.3355788168259352No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG52890.2349273808328751No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG52250.23208462182865802No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT47030.20889836870051268No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG45760.20325726880151945No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC40170.1784275456240611No Hit
GTAGCTGCCCTGGGTGTTATGGCAGAGACCATGGTTCATGCAGGGCTTGG36090.1603049569721774No Hit
CCCACAAGGCGTCCTCTTCATCCTTCAGCGTCTCCATGTAGTCATCCATC34280.15226527916337604No Hit
TCCTAGGTCAGCGGTGTACCATTGACATTGACGAGTGTATCTCCAAGCCC33740.14986670125356788No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA33070.14689068792102813No Hit
CAAACAGTTGTACAATCTCCTGGGAGGTCCATGACCTCTGCTTGCTCAGT32410.1439590926979293No Hit
CCTCGGTGTCCGAGGACCTGGGGCTGGTGCATTTTCGGTTGTTGCTGATC31990.14209353210141187No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA31570.14022797150489444No Hit
TGGTGGATACAGATGCGAGGTGAGGACACTGAGCAAGCAGAGGTCATGGA31540.14009471717657176No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA31260.1388510101122268No Hit
CCTGCTGCCAAGAGGGTCAAGTTGGACAGTGTCAGAGTCCTGAGACAGAT31110.1381847384706134No Hit
GGGGTTTATGTCTAAAAGGAGGAGGGCCAGAGATGGATGACTACATGGAG30800.13680777707794578No Hit
CTCCGGATGTGACACCTTGCTTTTGTACCATTAACTTCACACTGGAAAAG30600.13591941488912795No Hit
AGCACATTACTTGGAAACACAGACCTCAAATGACTTTTCCAGTGTGAAGT30490.13543081568527815No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT30260.13440919916813765No Hit
CGAAGTCCGTGTAGACGGTGTCCTTGAGCAGCGCCCCCGACCCGAAGTCG29330.13027831499013476No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT29280.13005622444293027No Hit
CCCGAAAAGCCGTCCAAGGGCTCCGGCTACCGCTGCATTCGCATCAACCA29170.12956762523908047No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT29060.12907902603523067No Hit
CAGACTCGACTCACCAGAGGCAGGCTGACCAACTCCTGCATTCGACTGTT28910.1284127543936173No Hit
CCATTAGCTCTGTTGTATCCCGGTGAGGGCAGCCTCTGGCTTGGGCAGGG28880.12827950006529462No Hit
GGCCTTCTTGCTGACAGGTATCATTTCTGTGAGAAGTGTTTCACAGAGAT28700.12747997409535858No Hit
GAAATTTCTCAGTGAGAACAAATGCCCTTTGGATGTGAAAGACAAGGTAA28680.1273911378764768No Hit
CCAAAGGGAAGGATGGGCTACATCACCCCCTTGCTTCTGCAGTCCAAACA28660.127302301657595No Hit
ACAAGTTCAGCAGCCCCAACAAGTACAGATGCAAGTTCAACCTCAACAGT28410.12619184892157273No Hit
AGATACCATCAACATCCTGCTGTATGATGACAACAGCATCATGACCTTCA28360.12596975837436827No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC28300.12570324971772293No Hit
GGGGCTGTGAAGGGTCGTCACCCAGGGTCACATTCTCGCCCTGGATCTCT28070.12468163320058241No Hit
AGAAAGTGGAGCATGTCATCAAAGATAGGCTGCAAGTCACAGGGGTCCAG27620.1226828182757423No Hit
CCCCAGGCACTGATACTCACCTGACTGAGGTTGAAGGTCATGATGCTGTT27500.1221498009624516No Hit
CCCTCTCTTCTCTGCAGAAAAGAAGGACACAGTGTTGCGGCAGGTACGCC27360.12152794743027912No Hit
CCACCTCCTGCTCTGCCTGAGGAGGGGTGAGAGCCAGCGATGGTGTCCTA27110.12041749469425683No Hit
ACCCCCATCTCTCCTCCTCCGCTTCTCCCTTGGCATCCCACAGCTCTAAA27110.12041749469425683No Hit
GACCCCATTGATCGACGGCCATGGACACAGCAGCACCTTGAAGCTGCAGA26790.1189961151921483No Hit
CAAGGACGAAAACATCCTTATCGACCTCAATCGCGGCGAGCTCAAGCTCA26780.11895169708270742No Hit
CCAGTGTGAAACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGG26710.11864077031662117No Hit
CTGTCCCTACACAGCAAGTTCATTCGTGTGCAGAATGAAGGAACTGGAAA26270.11668637350122195No Hit
ACCACCTCATGTTCCTCCTAAGAGAAGTGGCCTTACCTTGTCTTTCACAT25900.11504290345190896No Hit
CAAGTTTTTCTAAAGACCTTGGATTAACAGGTTGATTACTAATGCTGATG25820.11468755857638183No Hit
ACAGAGGCCCAGCTGTAGTTCTATTACCAAGTTTTGTACCTTTGTAGCCT25710.11419895937253202No Hit
GTTGTCGTCGTTGCAAATTCTGTCACGTTTGTGGAAGGCAACATCAGGCT25640.11388803260644578No Hit
GAACACACTGGCGGTTGTCAATTTCCAGAAAGACTTTAGAGCTGTGGGAT25250.11215572633825101No Hit
TGCCACCCTCTGGATTGAGCATCCACCAAGAACTTTTTCCAGTTCCTTCA25050.11126736414943318No Hit
ACAGCATTTGCCATCCACATACGCAAGAGCCGACTTAAGTGGGCAGTCAG24980.11095643738334693No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC24970.11091201927390605No Hit
CCTACAGGAGTACCTCTCTCTAGAACTGTACGCTGTACCTGCATCAGCAT24960.11086760116446517No Hit
GCCAATGCCCAGGACAACATGGGCCGCTGTCCACTCCATGCTGCAGTGGC24690.1096683122095611No Hit
CTTCTCATGTTCTTTACCTGGAAGACACCTTGGGCATCAGCTGCCACTGC24490.10877995002074325No Hit
CAAGGGCAAGTAGGGAGATTTGGCACCAGGTCCCCAATCTTCTTGTCAAT24270.10780275161304365No Hit
ACTCCCGCCTGCGGGAACTGGTACCCGGAGTCCCGAGAGGCACTCAGCTT24120.10713647997143028No Hit
GATCCCCATCAAGTACAAGGCAATGAAGAAAGAGTTCCCCAGCATCCTGA23900.10615928156373065No Hit
CGAGAATGTAGTCGATGACGCGCTGTAGGATTTCCACCTGGCTAAGCTGA23790.10567068235988085No Hit
CAGCCCTGCCAGAATGGAGGCACCTGTATTGACCTTGTGAACCATTTCAA23730.1054041737032355No Hit
GGTGCAGGGGTTGGTGTAGTCGCAGACAGTGATGAACCTCAGGATGCTGG23710.10531533748435373No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG23700.10527091937491283No Hit
ACCCCGAGTGCCTGGTGGGCAAGAGCACTTGAAATGGTTCACAAGGTCAA23590.10478232017106302No Hit
GCCAGAGCAGGGTTGAGCTTACCTGGAGAGAGGCTGAGTGCAAATTTGGT23550.10460464773329947No Hit
GCACAGGGGACTGCATCCATGATGGAAAAGTTAAGCACAATGGTCAGATT23540.10456022962385857No Hit
CGCCTTGTCTCCTACAGGGAAACCAATGAAAAGCGTGCTGGTGGTGGCTC23520.1044713934049768No Hit
ACCAAGGAATCTGCTTTTCAAGTATGCCACCAACAGTGAAGAGGTAAGCC23510.10442697529553589No Hit
GCCTGTTCTCTGCTTCCCCTTACCTAGGTATGACAAATACTGTGCAGACC23400.10393837609168609No Hit
AACCCCCAAGCTGTCTGTAGTCACACACAGGGAAGGGCAGGAGGCAGGGA23380.10384953987280429No Hit
GAAGTGGACTTTGCTATTAACGTATGCACTCTCCTATCAAATGAAAGCAA23300.10349419499727716No Hit
ATGCCTTAGACATGCTGGTGCTGTTCAGGGTATGGGGCCTGGGAGGTGAT23250.10327210445007272No Hit
CCATCCCAGAGCTCCTGTACAGTGACCTCCAGAGGAGCAGCAGTGAGCAA23220.10313885012175003No Hit
TACCTGCAGAGCAAGGAATGTGACTTCCAGACCAAATTTGCACTCAGCCT23210.10309443201230914No Hit
CTCCAGGCAGTGGCAGTGCTGGAAGCTTGAGTAGGCTCCATCCAGTCCCA23160.10287234146510468No Hit
CCAAATGGACTTGTGTTCCCAGTTATTCCCATCTGAAACAAAAAAGAGGT23080.10251699658957755No Hit
GAGCACACAGAGCACCTGTCATTTTCCAACACCCAAATCTGACCATTGTG22950.10193956116684598No Hit
CAAATGGTGGGAACAGACGAGATGCCTCCGGTGGTGGAGACATGGGTGAC22890.10167305251020062No Hit
GAGGACACTACACTTACTTATTGCAGTCAATGTGTAAAATCAAATGGGAA22810.10131770763467347No Hit
AAAGGCCTCCACATGCTCTGTGATGAGAGAGCATCGCATCGCTTGCTCAC22680.1007402722119419No Hit
CTCATCCATGCCTGAATGAGGGAACGTGTGTTGATGGCCTGGGTACCTAC22670.10069585410250101No Hit
CCAGACCCACTTCGAGAGCAAGACAGAGCTGCTGCCTTCCCGGCCTCACG22630.10051818166473744No Hit
CGCCTGTCTCCACCACCTGAGGAGTCACCCATGTCCCCTCCACCTGAAGA22630.10051818166473744No Hit
GGCAGTCGCGCAGGGGAAGTGGTAAAGCCGTGGACATCCAGGTGAGCGGC22610.10042934544585566No Hit
CAGAGTGAAACACCCACTTACTTGCAGCACTGCTTCTTTATATTGCGACC22570.1002516730080921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)