FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251334
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN361491.6056702381787864No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG74020.3287828460814788No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG54560.24234520510950397No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG49540.22004731417017645No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG45710.20303517825431502No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT45250.200991945220034No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC39960.17749476532580238No Hit
GTAGCTGCCCTGGGTGTTATGGCAGAGACCATGGTTCATGCAGGGCTTGG35240.1565294176697016No Hit
TCCTAGGTCAGCGGTGTACCATTGACATTGACGAGTGTATCTCCAAGCCC34530.15337573189939832No Hit
CCCACAAGGCGTCCTCTTCATCCTTCAGCGTCTCCATGTAGTCATCCATC32960.14640208871717833No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA32670.1451139635433925No Hit
TGGTGGATACAGATGCGAGGTGAGGACACTGAGCAAGCAGAGGTCATGGA32370.14378142026016577No Hit
CAAACAGTTGTACAATCTCCTGGGAGGTCCATGACCTCTGCTTGCTCAGT31970.1420046958825301No Hit
GGGGTTTATGTCTAAAAGGAGGAGGGCCAGAGATGGATGACTACATGGAG31790.14120516991259405No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA31590.14031680772377622No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT31530.14005029906713087No Hit
CCTCGGTGTCCGAGGACCTGGGGCTGGTGCATTTTCGGTTGTTGCTGATC31480.13982820851992642No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA31250.1388065920027859No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT30930.13738521250067737No Hit
AGCACATTACTTGGAAACACAGACCTCAAATGACTTTTCCAGTGTGAAGT30760.13663010464018222No Hit
CCTGCTGCCAAGAGGGTCAAGTTGGACAGTGTCAGAGTCCTGAGACAGAT30600.13591941488912795No Hit
CTCCGGATGTGACACCTTGCTTTTGTACCATTAACTTCACACTGGAAAAG29950.13303223777547No Hit
GGCCTTCTTGCTGACAGGTATCATTTCTGTGAGAAGTGTTTCACAGAGAT29250.12992297011460763No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC29180.12961204334852136No Hit
CCAAAGGGAAGGATGGGCTACATCACCCCCTTGCTTCTGCAGTCCAAACA29120.129345534691876No Hit
AGATACCATCAACATCCTGCTGTATGATGACAACAGCATCATGACCTTCA28900.1283683362841764No Hit
ACAAGTTCAGCAGCCCCAACAAGTACAGATGCAAGTTCAACCTCAACAGT28860.12819066384641284No Hit
CAGACTCGACTCACCAGAGGCAGGCTGACCAACTCCTGCATTCGACTGTT28730.12761322842368125No Hit
CGAAGTCCGTGTAGACGGTGTCCTTGAGCAGCGCCCCCGACCCGAAGTCG28720.12756881031424036No Hit
CCATTAGCTCTGTTGTATCCCGGTGAGGGCAGCCTCTGGCTTGGGCAGGG28380.12605859459325006No Hit
GAAATTTCTCAGTGAGAACAAATGCCCTTTGGATGTGAAAGACAAGGTAA28350.12592534026492735No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT28290.125658831608282No Hit
CAAGGACGAAAACATCCTTATCGACCTCAATCGCGGCGAGCTCAAGCTCA28240.12543674106107755No Hit
CCCGAAAAGCCGTCCAAGGGCTCCGGCTACCGCTGCATTCGCATCAACCA28160.12508139618555042No Hit
GGGGCTGTGAAGGGTCGTCACCCAGGGTCACATTCTCGCCCTGGATCTCT27700.12303816315126942No Hit
AGAAAGTGGAGCATGTCATCAAAGATAGGCTGCAAGTCACAGGGGTCCAG27550.12237189150965605No Hit
GACCCCATTGATCGACGGCCATGGACACAGCAGCACCTTGAAGCTGCAGA27490.1221053828530107No Hit
CCCTCTCTTCTCTGCAGAAAAGAAGGACACAGTGTTGCGGCAGGTACGCC27480.12206096474356981No Hit
TGCCACCCTCTGGATTGAGCATCCACCAAGAACTTTTTCCAGTTCCTTCA27000.11992889549040703No Hit
CCCCAGGCACTGATACTCACCTGACTGAGGTTGAAGGTCATGATGCTGTT26770.11890727897326651No Hit
CCACCTCCTGCTCTGCCTGAGGAGGGGTGAGAGCCAGCGATGGTGTCCTA26750.11881844275438472No Hit
ACCACCTCATGTTCCTCCTAAGAGAAGTGGCCTTACCTTGTCTTTCACAT26250.11659753728234015No Hit
GAACACACTGGCGGTTGTCAATTTCCAGAAAGACTTTAGAGCTGTGGGAT26250.11659753728234015No Hit
GTTGTCGTCGTTGCAAATTCTGTCACGTTTGTGGAAGGCAACATCAGGCT25930.11517615778023163No Hit
ACAGCATTTGCCATCCACATACGCAAGAGCCGACTTAAGTGGGCAGTCAG25900.11504290345190896No Hit
ACCCCCATCTCTCCTCCTCCGCTTCTCCCTTGGCATCCCACAGCTCTAAA25900.11504290345190896No Hit
CCAGTGTGAAACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGG25770.11446546802917736No Hit
ACAGAGGCCCAGCTGTAGTTCTATTACCAAGTTTTGTACCTTTGTAGCCT25600.1137103601686822No Hit
CAAGTTTTTCTAAAGACCTTGGATTAACAGGTTGATTACTAATGCTGATG25500.11326617907427329No Hit
GCCAGAGCAGGGTTGAGCTTACCTGGAGAGAGGCTGAGTGCAAATTTGGT25240.11211130822881012No Hit
CCTACAGGAGTACCTCTCTCTAGAACTGTACGCTGTACCTGCATCAGCAT24940.11077876494558338No Hit
GCCAATGCCCAGGACAACATGGGCCGCTGTCCACTCCATGCTGCAGTGGC24680.10962389410012019No Hit
CGAGAATGTAGTCGATGACGCGCTGTAGGATTTCCACCTGGCTAAGCTGA24660.1095350578812384No Hit
CTTCTCATGTTCTTTACCTGGAAGACACCTTGGGCATCAGCTGCCACTGC24660.1095350578812384No Hit
CTGTCCCTACACAGCAAGTTCATTCGTGTGCAGAATGAAGGAACTGGAAA24550.1090464586773886No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG24530.10895762245850683No Hit
CAAGGGCAAGTAGGGAGATTTGGCACCAGGTCCCCAATCTTCTTGTCAAT24300.10793600594136632No Hit
ACCCCGAGTGCCTGGTGGGCAAGAGCACTTGAAATGGTTCACAAGGTCAA23840.1058927729070853No Hit
GATCCCCATCAAGTACAAGGCAATGAAGAAAGAGTTCCCCAGCATCCTGA23770.10558184614099908No Hit
GAAGTGGACTTTGCTATTAACGTATGCACTCTCCTATCAAATGAAAGCAA23720.10535975559379461No Hit
CAGCCCTGCCAGAATGGAGGCACCTGTATTGACCTTGTGAACCATTTCAA23650.10504882882770837No Hit
ACCAAGGAATCTGCTTTTCAAGTATGCCACCAACAGTGAAGAGGTAAGCC23520.1044713934049768No Hit
AACCCCCAAGCTGTCTGTAGTCACACACAGGGAAGGGCAGGAGGCAGGGA23500.104382557186095No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC23430.10407163042000875No Hit
GGTGCAGGGGTTGGTGTAGTCGCAGACAGTGATGAACCTCAGGATGCTGG23400.10393837609168609No Hit
GCACAGGGGACTGCATCCATGATGGAAAAGTTAAGCACAATGGTCAGATT23270.10336094066895449No Hit
ACTCCCGCCTGCGGGAACTGGTACCCGGAGTCCCGAGAGGCACTCAGCTT23230.10318326823119094No Hit
CAAATGGTGGGAACAGACGAGATGCCTCCGGTGGTGGAGACATGGGTGAC23210.10309443201230914No Hit
GGCTTACTCAAAATTGGTGGTGTAGGAGGATGCAGAGGAGTTGGTGAGTC23170.10291675957454559No Hit
GAGCACACAGAGCACCTGTCATTTTCCAACACCCAAATCTGACCATTGTG23100.10260583280845934No Hit
AAAGGCCTCCACATGCTCTGTGATGAGAGAGCATCGCATCGCTTGCTCAC22920.10180630683852329No Hit
ATGCCTTAGACATGCTGGTGCTGTTCAGGGTATGGGGCCTGGGAGGTGAT22900.10171747061964152No Hit
CGCCTTGTCTCCTACAGGGAAACCAATGAAAAGCGTGCTGGTGGTGGCTC22820.10136212574411438No Hit
CCATCCCAGAGCTCCTGTACAGTGACCTCCAGAGGAGCAGCAGTGAGCAA22810.10131770763467347No Hit
GAGGACACTACACTTACTTATTGCAGTCAATGTGTAAAATCAAATGGGAA22810.10131770763467347No Hit
GCCTGTTCTCTGCTTCCCCTTACCTAGGTATGACAAATACTGTGCAGACC22760.10109561708746904No Hit
CCCAGGCCCTTCCTTTTATATTGACTGCGTCACCACAGCTGATGTTACCT22740.10100678086858725No Hit
CTCCAGGCAGTGGCAGTGCTGGAAGCTTGAGTAGGCTCCATCCAGTCCCA22700.10082910843082367No Hit
CAATTACCTGACAGTTTTTCCCAGTGTAGCCCAGGGGGCAGCTGCAGCGG22570.1002516730080921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)