FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004965484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237503
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN70959557.34087109283775No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCCAAGTCTATCTCGTATGC265452.1450453049406746No Hit
AGGAGGGAGCAAAGGAGTCGAGGTATATCTCCCGGTT175061.4146228332375759No Hit
AACCGGGAGATATACCTCGACTCCTTTGCTCCCTCCT170271.3759158563656009No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAGTCTATCTCGTAT69050.557978445304779No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC50570.4086454739907701No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG49250.39797883318262667No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG34690.28032255275340745No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG34490.2787063950552039No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAGTCTATCTCGTATGCCG27940.2257772304390373No Hit
TCCGACAGGCAGCACACCGCCTCGTAGCAGCCGCGCACCGGGCTCAGCGC22760.18391874605556513No Hit
TTATACACATCTCCGAGCCCACGAGACCCAAGTCTATCTCGTATGCCGTC22150.17898946507604427No Hit
GGGGCACCTCTGGACTCACTCCCCAGCATGAAGGCGCTGAGCCCGGTGCG21640.17486826294562519No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAGTCTATCTCGTATGCC19940.16113092251089492No Hit
AAAGGCCTCCACATGCTCTGTGATGAGAGAGCATCGCATCGCTTGCTCAC18140.1465855032270629No Hit
CGAAGTCCGTGTAGACGGTGTCCTTGAGCAGCGCCCCCGACCCGAAGTCG16550.13373704952634458No Hit
CCATCCCAGAGCTCCTGTACAGTGACCTCCAGAGGAGCAGCAGTGAGCAA16480.13317139433197334No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAGTCTATCTCGTATGCCGT16440.13284816279233264No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA16020.12945423162610514No Hit
CAAGGATAAGGGTGACAGCAACAGCTCGGCGGGCTGGAAGGTGAGTGGAC15890.12840372912227282No Hit
CCAGGACCCTGCTGCTGTTGCTGCTGGATTGCCACCTGTCCTAGAAGGTG15690.12678757142406927No Hit
CAAGGACGAAAACATCCTTATCGACCTCAATCGCGGCGAGCTCAAGCTCA15510.1253330294956861No Hit
CCCGAAAAGCCGTCCAAGGGCTCCGGCTACCGCTGCATTCGCATCAACCA15300.12363606391257234No Hit
GGCCCAGAAACTGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCACC14880.12024213274634486No Hit
GGCGAGGGGAAGGAGGGGGCTCCAGGGGGCGGCATGTAGTGGTGCACCAG14860.1200805169765245No Hit
CCGGCATTTCTTGGCGGTCCTGTCTGTCTCTTAACACATCTCCGAGCCCA14790.11951486178215324No Hit
GCACAGCTGCGCCCTCGCCTGACCCACCGCGCCCCCAAGGTCCGGCCGCG14680.11862597504814129No Hit
CCCCGGAGCACGCAGCCCGTGCCCCGCTTCGACTGCGTGCTCAAGCTGGT14660.11846435927832094No Hit
ACTCCCGCCTGCGGGAACTGGTACCCGGAGTCCCGAGAGGCACTCAGCTT14550.11757547254430899No Hit
GACGCCGATGAAGGCGTACAAGAAAGGGTTGACGCAGCAGCGGACGCAGG14530.11741385677448862No Hit
CGAGAATGTAGTCGATGACGCGCTGTAGGATTTCCACCTGGCTAAGCTGA14470.11692900946502756No Hit
GCACGGCATCTTCTTCCGCTCACTCGATGAGACCACGCTCCGGCCACTGG14310.11563608330646472No Hit
CACCAAAGCAGTGCAGCAGGCAGACCACGCTGAGGACCCCCAGGGCCCGC14110.11401992560826114No Hit
AAGCAACTCAACATCGCCTACGACGTCACCTACAGCCTGGCCTGCGTCCG13810.11159568906095581No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAGTCTATCTCGTATG13620.11006033924766243No Hit
ACAGGACCGCCAAGAAATGCCGGCTGTCTCTATACACATCTCCGAGCCCA13550.10949468405329119No Hit
GTGGCTGGGCACTCGCGCAGAGGCCGGCCCGACGAGCCATGGTTGCTGGG13530.10933306828347082No Hit
GACCTGTCCCAGAAGCTGTATGCCACCATGGAGAAGCACAAGGAGGTAGG13340.10779771847017744No Hit
CCCTCCTCCCGGCCAGAGGCACGGCTGCAGCACCGCAGCCCACGCCTACC13320.10763610270035709No Hit
CGCAGGCAGTCAATGGCTCCCAGTTCCTGCCGCTGACCGGTCTGCAGGTG12980.10488863461341105No Hit
CAGCCTGGCGGGGGACGCGGGGAGAATGTGGACTGGGTAGAGATGAACGA12780.10327247691520748No Hit
ACAGGACCGCCAAGAAATGCCGGCTGTCTCTTAACACATCTCCGAGCCCA12660.10230278229628534No Hit
GCTCGTGGTAGAGGTCCAGCTTATTGTGGGACAGGTCTAGCACCTGCAGA12430.10044420094335124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)