FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004965485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237503
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN69745956.36018660156783No Hit
TCTCTTATACACATCTGACGCTGCCGACGACTAGCGCTGTGTAGATCTCG279752.2606005803622296No Hit
AACCGGGAGATATACCTCGACTCCTTTGCTCCCTCCT171701.3874713839077562No Hit
AGGAGGGAGCAAAGGAGTCGAGGTATATCTCCCGGTT168211.3592694320741041No Hit
TGTCTCTTATACACATCTGACGCTGCCGACGACTAGCGCTGTGTAGATCT71100.5745440617113655No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG48630.39296874431819556No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC48500.3919182418143633No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG34280.2770094294720902No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG33510.2707872223340065No Hit
GGGGCACCTCTGGACTCACTCCCCAGCATGAAGGCGCTGAGCCCGGTGCG22470.18157531739316995No Hit
TCCGACAGGCAGCACACCGCCTCGTAGCAGCCGCGCACCGGGCTCAGCGC21380.17276725793796055No Hit
CCGGCATTTCTTGGCGGTCCTGTCTGTCTCTTAACACATCTGACGCTGCC19120.15450467594826034No Hit
ACAGGACCGCCAAGAAATGCCGGCTGTCTCTTAACACATCTGACGCTGCC17480.14125218282299115No Hit
CCATCCCAGAGCTCCTGTACAGTGACCTCCAGAGGAGCAGCAGTGAGCAA17340.14012087243424864No Hit
AAAGGCCTCCACATGCTCTGTGATGAGAGAGCATCGCATCGCTTGCTCAC17110.13826229108131455No Hit
CAAGGACGAAAACATCCTTATCGACCTCAATCGCGGCGAGCTCAAGCTCA16220.1310703893243087No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA16110.13018150259029676No Hit
CGAAGTCCGTGTAGACGGTGTCCTTGAGCAGCGCCCCCGACCCGAAGTCG15700.12686837930897946No Hit
GCACAGCTGCGCCCTCGCCTGACCCACCGCGCCCCCAAGGTCCGGCCGCG15640.12638353199951838No Hit
GGCCCAGAAACTGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCACC15400.1244441427616741No Hit
ACAGGACCGCCAAGAAATGCCGGCTGTCTCTATACACATCTGACGCTGCC15300.12363606391257234No Hit
CCCCGGAGCACGCAGCCCGTGCCCCGCTTCGACTGCGTGCTCAAGCTGGT15050.12161586678981788No Hit
CCAGGACCCTGCTGCTGTTGCTGCTGGATTGCCACCTGTCCTAGAAGGTG15030.12145425101999753No Hit
CCCGAAAAGCCGTCCAAGGGCTCCGGCTACCGCTGCATTCGCATCAACCA15010.12129263525017717No Hit
GACGCCGATGAAGGCGTACAAGAAAGGGTTGACGCAGCAGCGGACGCAGG14820.11975728543688378No Hit
CAAGGATAAGGGTGACAGCAACAGCTCGGCGGGCTGGAAGGTGAGTGGAC14810.11967647755197362No Hit
CGAGAATGTAGTCGATGACGCGCTGTAGGATTTCCACCTGGCTAAGCTGA14570.11773708831412935No Hit
ACTCCCGCCTGCGGGAACTGGTACCCGGAGTCCCGAGAGGCACTCAGCTT14360.1160401227310156No Hit
GGCGAGGGGAAGGAGGGGGCTCCAGGGGGCGGCATGTAGTGGTGCACCAG14280.11539365965173418No Hit
CACCAAAGCAGTGCAGCAGGCAGACCACGCTGAGGACCCCCAGGGCCCGC13960.11280780733460849No Hit
GTGGCTGGGCACTCGCGCAGAGGCCGGCCCGACGAGCCATGGTTGCTGGG13790.11143407329113546No Hit
GCACGGCATCTTCTTCCGCTCACTCGATGAGACCACGCTCCGGCCACTGG13650.11030276290239296No Hit
CCCTCCTCCCGGCCAGAGGCACGGCTGCAGCACCGCAGCCCACGCCTACC13330.10771691058526728No Hit
AAGCAACTCAACATCGCCTACGACGTCACCTACAGCCTGGCCTGCGTCCG13210.10674721596634514No Hit
GACCTGTCCCAGAAGCTGTATGCCACCATGGAGAAGCACAAGGAGGTAGG13170.10642398442670441No Hit
CAGCCTGGCGGGGGACGCGGGGAGAATGTGGACTGGGTAGAGATGAACGA12910.1043229794190398No Hit
CGCAGGCAGTCAATGGCTCCCAGTTCCTGCCGCTGACCGGTCTGCAGGTG12840.10375732422466856No Hit
GCTCGTGGTAGAGGTCCAGCTTATTGTGGGACAGGTCTAGCACCTGCAGA12580.10165631921700392No Hit
GCACCACTCTGCATTTGCCTAGCCTGCCTTCCTCTAACTGTGCTCTCCCT12420.10036339305844107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)