FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219152
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN112670.507716461062604No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG106110.47815561980432164No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG94980.42800132663287593No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG72530.32683655738768685No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT66520.2997541403202665No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT63820.28758733065603437No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA49860.22468041846615286No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG38570.17380512916645638No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG37270.16794703562441868No Hit
AGAAAGGGGTTTGTGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCAC34900.15726728047470384No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC34760.1566364088624844No Hit
GTGATGTCTCGATTGGCAAAATGGTCTAACAGGATCTTGGCTGCTTCATA34560.15573516370217091No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA34390.15496910531590447No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT33940.15294130370519912No Hit
CTGTAGGAAGAGACACCTCTGTTTCTTGCTGCCCGGGAGGGGAGCTATGA33770.15217524531893264No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG31890.14370354081198583No Hit
ATCGCCTCTATGTGTCTGTGAAGGAGATTGGTGGATTGACTCAGGTGAGT31890.14370354081198583No Hit
CAGCTTTCTATCACATAGTGAAGGACGACGCATTGTCGTTATCTGCGGTC31090.14009856017073188No Hit
CAAGAGGCACACGCTGCAGACCCACAGTGACAAACCCTACAAGTGTGACC30620.1379806340439952No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT30560.13771026049590113No Hit
TTGTAAGTTCGAAATGGGCACAGTTTGTACTCCCCCTGAGGGTGGCATTG30450.1372145756577287No Hit
CGATACACTTTGCCCCATTCAGACACGGAGTACTGGAACAGTCATCAATA30120.13572752114321146No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC30030.1353219608210704No Hit
CTCCTGTGCCGGGAGGTACTCAGAAAACATTTCTCGGCAGACCAGTGAGA29010.1307256105034716No Hit
GTGGCTGGCGAGGTTGCCCTTGTAGCGGAAGGAGGCCTGGCAGCGGTCAC28990.13063548598744024No Hit
GACTCATACCTCCATAACTCGACTTATTGTCCTGAAGTGTTTGTATAGGC28720.12941880502101705No Hit
CCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGGTGAGTAGCTGC28600.12887805792482895No Hit
CAAATGTTATCTGTGAAAAGGCAGCAACAGCAGCAACATTCACCAGCACC28500.1284274353446722No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC28310.12757125244237438No Hit
GGATGGGCTGGTGCCTATTGTGACGTGCCCAATGTCTCTTGTGACATAGC28160.12689531857213926No Hit
CACCCAGCCCAAACTACCAAAAATATACATATGGCCAATCCAGCATGAGC28090.12657988276602955No Hit
AGCAGCACCAGCAATCTCAAGACCCACCTGCGACTCCATTCTGGAGAGAA27960.12599407341182578No Hit
GGACAGTCAATACTGCAGTGAATGACCTGAGCCCAAGGGAGCAAAGCTTA27850.12549838857365336No Hit
TTTGAGGTTTCACTGGGCCAGTTTGCCAGATTGATATTGATGACTGTTCC27280.12292983986675991No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG27230.12270452857668153No Hit
GGGTGAACTTGGCAGGGCACACCTTGCATTGGTATGGTTTCTCTCCAGAA27000.12166809664232103No Hit
GGACTTTCAGGTGAATCCCTGACTCCAGAACGCACTCAGCTCCTCTATCT26680.12022610438581945No Hit
ACCACCACACTCACCTTTCCACGGTCATCTTGATGGTAGCTGGGACATAA26520.11950510825756866No Hit
ACAAAGTCCATAATGTCTGAAGGCAGGATATTCCCACAGTCATCACTGTT26490.11936992148352163No Hit
ACACGGATGGCTTTGTGCCTTTTAGAATTCTGAGCTTGCACCAAGGTTTT26470.11927979696749029No Hit
ATCCACACACTGCCCATTGTTCACACAAGGGTTGCTCTGACATTCATTTA26440.11914461019344326No Hit
GTGGTGTCGGAAACATACGTGTGAGGAGATTCTAGGGAATCAACAGGGGA26430.1190995479354276No Hit
GAGAAGGTCCAACTGTCTGAGAGTTCAGTAACTTTATCCCCTGTTGATTC26420.1190544856774119No Hit
CAGTGCATGGATAGGATTGGAGGCTACAGTTGTCGCTGCTTGCCTGGCTT26400.11896436116138057No Hit
CTTTCAGCCAATAGCAATCCTTGTGAGCATGCAGGAAAATGTGTGAACAC26300.11851373858122383No Hit
AGGACATCTCTGATTGGAGGGGGGACTCTTGCTCATAGGAGATGATGGTC26300.11851373858122383No Hit
CTCTGAAATCACCTGTTAACGATGTTATTGAGCCGGCTGGCTTGTGGGAT26270.1183785518071768No Hit
TGTAAGCCAGCCAGCTCAACAAGGTCAAACTTATGCACCAGCCATTCACC26030.1172970576148006No Hit
CTGCCGAAGCCTTGGCGTTCTCAGGTGAGTGCAGGGTTTGCTCCTGGAGG26010.11720693309876926No Hit
GTTTAGGTTTCAAAGGTGTGCATTGTGAATTAGAAATAAATGAATGTCAG25760.11608037664837739No Hit
TGCTCTGCAGGTAATCATCAGAGACAACCACCACCATCCGGCGGCACCTA25570.11522419374607959No Hit
CAATACCACAATAACCAGGGTACCTTCTGCCAGGTTCTCAGGTTGGTCAG25470.11477357116592285No Hit
GACCTCCAGTCCCCAACCAAGCTGAGTGCCAGCGGGGAGGACTCCACCAT25440.11463838439187582No Hit
GGGGCTGCTGAACTTGTACTTGTACCTGCTGTGGTGGGGGTGCTGGTGAA25430.11459332213386013No Hit
CATCAACACCATCCAACTGTGAGATTTTTGGAAGATCACACTGTTCCTCC25370.1143229485857661No Hit
CTCACAACGAGGTCCTGCATAACCCTTCAGACACTCACAGTGGAAGGCGC25330.1141426995537034No Hit
GGACACCTATAATACTGAGCTCCTGAAATCAGATTCAGACAATAACAACA25200.11355689019949963No Hit
ACTTGAAAAGCAGATTCCTTGGTAAGGATGCCTCCGCCTGAACCGAAGTA25110.11315132987735856No Hit
AGAGCAAGGTCATGGCAACAATCAGGATTTAACTAGGAACAGTAGCACCC24800.11175439987887266No Hit
GGCATCTAGATCAGTTACTCGGTTGCGAATCAGAATCTAGAAGAGGAGAAAGTACAGAAAAGAGAGTCACCTGG24770.11161921310482563No Hit
GGGACTGCTGAGACTGGGGCTGAGGATACGCAGCCTGCTGGGAGAGCGTC24760.11157415084680997No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG24670.11116859052466889No Hit
CAGTACCTTTGGTCTAACAGAGGCAGAAGAGTTGGATTCTGTTGAGTTCA24550.11062784342848078No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT24500.11040253213840243No Hit
GGAGAGGAACGACTGGCATCCCATAATTTATTTCGGGAGGAGGAACAGTG24470.1102673453643554No Hit
CTGGGTCCTGTCGATACAGTGCTTCTAGGGTTGGCATGAGGGCCTGGCGT24420.11004203407427701No Hit
ATGTTAGGTTGTCCAGTTAGCTGAACTGTCTGACCAGCTGGAAGAGCTGC24340.10968153601015163No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG24270.10936610020404189No Hit
TTTGTCCTCTTTGTCAAATGTCACGACTTCGGCCACACAGTTAGGGGCAC24230.1091858511719792No Hit
ACTACAAGCCAACTGGCTTAACACAGGTCAATAAGAAACTATCACATGGG24150.10882535310785381No Hit
GAACCAATCAAGCATTGCCCAAGATCGAGGTGAGAGCCTGGGTGTTGGGG24140.10878029084983815No Hit
CCAGGCTGCCCTCAGAGCTGCAGGGGTTGGAGAGGCACTCGTTGATGTCT24100.10860004181777543No Hit
GATGTTCATGATATTTTGTTACACGTTCATTGTCAAAACCTTGGTGCAAG24090.10855497955975976No Hit
CGGTGCGCAGCCGCCGGTACTGAGATGACTTGGAGTGCACAAACTGCTTG24070.10846485504372842No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC24020.10823954375365004No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG23920.1077889211734933No Hit
CCCTGACTCTGTCCCACTGCCCAGGTACATTAACCATTCCTGTGCCCCTA23910.10774385891547762No Hit
GGGCACATACTTCTGGTTCTGGATGGGAACAGTTGGGGAAACAGGTACCT23880.1076086721414306No Hit
GTGACCAGGGGTGCAACAGTGAGGAGTGTGGTTGGGATGGGCTGGACTGT23870.10756360988341493No Hit
TTTCTACAACCAGCTTGCATTTTTTCTGCCCACAATGAGCGGGGTAAGAT23820.10733829859333655No Hit
GAACTTGTATTTCTTCCAGTTGCAGGCTTTGGGGTCAGTGGGGCTCTTGG23770.10711298730325819No Hit
GAACAGCACCAGCATGTCTAAGGCATATCAGAGCACCTTCACAGGCGAGA23760.1070679250452425No Hit
CCATAACAGCTTTTTTCTCTCCTTGAAGTATTTGTCTGAAAGTGGCCACC23710.10684261375516414No Hit
CAAAGCGCCGTAGCTCCCGCTGCCCGTGTAGACTGGGCTCCCCGCAGTTA23640.10652717794905442No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT23610.10639199117500739No Hit
CGTGCTAATTGCATTAACCTGCCTGGATGGTACCACTGTGAGTGCAGAGA23610.10639199117500739No Hit
GCCCTCGAGGCCACGGGATTCTTCATCTGCAATATCTGGACCATCATCTC23490.1058512440788193No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA23480.10580618182080362No Hit
ATGTGCCCTGTGCACCCTCACCTTGTGTCAATGGAGGCACCTGTCGGCAG23430.10558087053072526No Hit
CCAGGTGACTCTCTTTTCTGTACTTTCTCCTCTTCTAGATTCTGATTCGCAACCGAGTAACTGATCTAGATGCC23400.10544568375667823No Hit
AACACCCAGGGCAGCTACATGTGTGAATGTCCACCAGGCTTCAGTGGTAT23330.1051302479505685No Hit
GCCCAAAGATGACAGAGCTGGCAGCAGAGGCTGGCCTCCTGCGGTGCCTC23280.10490493666049014No Hit
GCATTTTAAGACTCTTACCATGTCCTTCATCACACAATCTACATCTTCTG23240.10472468762842743No Hit
CCGAAAGAAGGACAGGGACTATTTGGATAAAAACTCTGAGAAGAGGAAAG23240.10472468762842743No Hit
GTGGACAGAGGCTGAGTTTGAAGAGAAGTGTACATACATTGTGAACGACC23170.10440925182231772No Hit
CTAACCTTCATGAAATGCAGCCTTTGGCACATGGGGCCAGCACTGTGCTT23160.10436418956430203No Hit
GTCCACAGTGGGGGTCCGGTGCGGCGTTGTGCTCTCTGTAACTGCGGGGA23110.10413887827422368No Hit
GTTTTCTCTGCAGTCTTTAAACCAGAGGAGTTACGCCAGGCCCTCATGCC23090.10404875375819231No Hit
GAACCCAAATTATACCCCTTGCCAAAAATAAAAATCTTACCCCGCTCATT23030.10377838021009826No Hit
AGAAGGAGGAGCTGCTGTTGGTATAGTGCTGAGGGTTCCAATGTTACCAG23030.10377838021009826No Hit
AGGGCACTCAAATGTCTGCCCTAGCTCCCTGCCCCTCCCCAACACCCAGG22920.10328269537192585No Hit
ACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGT22880.10310244633986315No Hit
CTGTAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAG22700.10229132569558103No Hit
ACCCCCAGCAGAATAATAAACAGAATGATGACAACAGCAACAGCAAGGAG22690.10224626343756534No Hit
CGAGACACAAAGCATGGATGAGATGTTGAGTACTGGCAGTCCTCTCCTGT22690.10224626343756534No Hit
TGATGTTGTTCTCACAATGGACTCCATCAAATCCTGCCTGGCACTTGCAA22650.10206601440550264No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG22630.10197588988947129No Hit
CACAGAAAAATCTGTAGCCTCCTCTGAAAGTGACCCAGCACTGCTGAGCC22580.10175057859939292No Hit
GTGAATCCTGGTGAGCAGACACAACTGTAGCGATTAATGCCATCCATACA22550.1016153918253459No Hit
CCTGTGTAGTTCCGCCACTTGCCAATGCCTTTCCACTGGAAGCAGGAAGA22530.10152526730931456No Hit
CCTGCTCATCAATGACCTTACACTGACGCAGGTAGGGCGTGAGCTTGGCA22470.10125489376122049No Hit
GACCACACACCTTGGCTTATGCCTCCAACAATCCCCTTAGCATCACCATT22420.10102958247114213No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG22410.10098452021312646No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT22390.1008943956970951No Hit
GAAGGATGAAGAGGACGCCTTGTGGGAGAATGTGGAGTGTAACCGGCACA22370.10080427118106376No Hit
CTTCCAGGGGTTAATTGTGAAATTAATTTTGATGACTGTGCAAGTAACCC22310.10053389763296972No Hit
GACCTTCACACGATTCTCCACTTGGTGAAAACATCCCATTGTCATGGTAG22280.10039871085892269No Hit
GACAAAGCAAGGTCAAGTATCCCCTCTGGTCCCTGCTGGTACCTCCAGGA22200.1000382127947973No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)