FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219152
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN110360.49730707946098335No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG106580.4802735459310583No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG98950.4458910430650987No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG70200.3163370512700347No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT63410.2857397780773917No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT62520.2817292371139967No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA49030.22094025105085188No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG38560.1737600669084407No Hit
AGAAAGGGGTTTGTGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCAC36980.16664023014196414No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG36820.16591923401371333No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA34820.15690678241057845No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC34700.15636603531439036No Hit
GTGATGTCTCGATTGGCAAAATGGTCTAACAGGATCTTGGCTGCTTCATA34080.15357217531741854No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT33640.15158943596472887No Hit
CTGTAGGAAGAGACACCTCTGTTTCTTGCTGCCCGGGAGGGGAGCTATGA33400.1505079417723527No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC30990.13964793759057514No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG30300.1365386417874936No Hit
CGATACACTTTGCCCCATTCAGACACGGAGTACTGGAACAGTCATCAATA30100.1356373966271801No Hit
CTCCTGTGCCGGGAGGTACTCAGAAAACATTTCTCGGCAGACCAGTGAGA29820.13437565340274124No Hit
CAGCTTTCTATCACATAGTGAAGGACGACGCATTGTCGTTATCTGCGGTC29810.13433059114472554No Hit
TTGTAAGTTCGAAATGGGCACAGTTTGTACTCCCCCTGAGGGTGGCATTG29690.13378984404853747No Hit
CACCCAGCCCAAACTACCAAAAATATACATATGGCCAATCCAGCATGAGC29550.13315897243631802No Hit
GTGGCTGGCGAGGTTGCCCTTGTAGCGGAAGGAGGCCTGGCAGCGGTCAC29280.1319422914698948No Hit
ATCGCCTCTATGTGTCTGTGAAGGAGATTGGTGGATTGACTCAGGTGAGT29150.13135648211569104No Hit
CAAGAGGCACACGCTGCAGACCCACAGTGACAAACCCTACAAGTGTGACC28930.1303651124393462No Hit
AGCAGCACCAGCAATCTCAAGACCCACCTGCGACTCCATTCTGGAGAGAA28710.12937374276300134No Hit
GTTTAGGTTTCAAAGGTGTGCATTGTGAATTAGAAATAAATGAATGTCAG28670.12919349373093866No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC28630.12901324469887596No Hit
CCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGGTGAGTAGCTGC28010.12621938470190414No Hit
GGACAGTCAATACTGCAGTGAATGACCTGAGCCCAAGGGAGCAAAGCTTA28010.12621938470190414No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT27850.12549838857365336No Hit
GGATGGGCTGGTGCCTATTGTGACGTGCCCAATGTCTCTTGTGACATAGC27150.12234403051255614No Hit
GGACACCTATAATACTGAGCTCCTGAAATCAGATTCAGACAATAACAACA27130.1222539059965248No Hit
CAAATGTTATCTGTGAAAAGGCAGCAACAGCAGCAACATTCACCAGCACC27110.12216378148049345No Hit
GTGGTGTCGGAAACATACGTGTGAGGAGATTCTAGGGAATCAACAGGGGA27060.12193847019041507No Hit
ACACGGATGGCTTTGTGCCTTTTAGAATTCTGAGCTTGCACCAAGGTTTT26930.1213526608362113No Hit
TGCTCTGCAGGTAATCATCAGAGACAACCACCACCATCCGGCGGCACCTA26910.12126253632017996No Hit
TTTGAGGTTTCACTGGGCCAGTTTGCCAGATTGATATTGATGACTGTTCC26740.12049647793391349No Hit
ACCACCACACTCACCTTTCCACGGTCATCTTGATGGTAGCTGGGACATAA26590.11982054406367837No Hit
GACTCATACCTCCATAACTCGACTTATTGTCCTGAAGTGTTTGTATAGGC26480.11932485922550595No Hit
GACCTCCAGTCCCCAACCAAGCTGAGTGCCAGCGGGGAGGACTCCACCAT26450.11918967245145894No Hit
GGAGAGGAACGACTGGCATCCCATAATTTATTTCGGGAGGAGGAACAGTG26380.11887423664534921No Hit
CTTTCAGCCAATAGCAATCCTTGTGAGCATGCAGGAAAATGTGTGAACAC26050.11738718213083195No Hit
GGGTGAACTTGGCAGGGCACACCTTGCATTGGTATGGTTTCTCTCCAGAA25990.1171168085827379No Hit
GGGGCTGCTGAACTTGTACTTGTACCTGCTGTGGTGGGGGTGCTGGTGAA25930.11684643503464387No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT25850.11648593697051847No Hit
CCAGGCTGCCCTCAGAGCTGCAGGGGTTGGAGAGGCACTCGTTGATGTCT25530.1150439447140169No Hit
GAGAAGGTCCAACTGTCTGAGAGTTCAGTAACTTTATCCCCTGTTGATTC25370.1143229485857661No Hit
AGGACATCTCTGATTGGAGGGGGGACTCTTGCTCATAGGAGATGATGGTC25320.11409763729568773No Hit
CTCTGAAATCACCTGTTAACGATGTTATTGAGCCGGCTGGCTTGTGGGAT25200.11355689019949963No Hit
CTCACAACGAGGTCCTGCATAACCCTTCAGACACTCACAGTGGAAGGCGC25050.11288095632926451No Hit
ATGTTAGGTTGTCCAGTTAGCTGAACTGTCTGACCAGCTGGAAGAGCTGC24970.11252045826513912No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG24930.11234020923307643No Hit
TGTAAGCCAGCCAGCTCAACAAGGTCAAACTTATGCACCAGCCATTCACC24750.11152908858879428No Hit
ACAAAGTCCATAATGTCTGAAGGCAGGATATTCCCACAGTCATCACTGTT24730.11143896407276294No Hit
CAATACCACAATAACCAGGGTACCTTCTGCCAGGTTCTCAGGTTGGTCAG24680.11121365278268455No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT24630.1109883414926062No Hit
GGACTTTCAGGTGAATCCCTGACTCCAGAACGCACTCAGCTCCTCTATCT24580.11076303020252781No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG24540.11058278117046512No Hit
CAGTGCATGGATAGGATTGGAGGCTACAGTTGTCGCTGCTTGCCTGGCTT24500.11040253213840243No Hit
ACTACAAGCCAACTGGCTTAACACAGGTCAATAAGAAACTATCACATGGG24500.11040253213840243No Hit
CCCTGACTCTGTCCCACTGCCCAGGTACATTAACCATTCCTGTGCCCCTA24410.10999697181626135No Hit
GTGGACAGAGGCTGAGTTTGAAGAGAAGTGTACATACATTGTGAACGACC24410.10999697181626135No Hit
AGAGCAAGGTCATGGCAACAATCAGGATTTAACTAGGAACAGTAGCACCC24370.10981672278419866No Hit
GAACCCAAATTATACCCCTTGCCAAAAATAAAAATCTTACCCCGCTCATT24360.10977166052618297No Hit
CTGCCGAAGCCTTGGCGTTCTCAGGTGAGTGCAGGGTTTGCTCCTGGAGG24360.10977166052618297No Hit
GTGACCAGGGGTGCAACAGTGAGGAGTGTGGTTGGGATGGGCTGGACTGT24280.10941116246205758No Hit
ATCCACACACTGCCCATTGTTCACACAAGGGTTGCTCTGACATTCATTTA24260.10932103794602623No Hit
GCCCTCGAGGCCACGGGATTCTTCATCTGCAATATCTGGACCATCATCTC24040.10832966826968139No Hit
CGGTGCGCAGCCGCCGGTACTGAGATGACTTGGAGTGCACAAACTGCTTG24040.10832966826968139No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG24030.10828460601166573No Hit
ACCCCCAGCAGAATAATAAACAGAATGATGACAACAGCAACAGCAAGGAG24020.10823954375365004No Hit
GGGCACATACTTCTGGTTCTGGATGGGAACAGTTGGGGAAACAGGTACCT24000.1081494192376187No Hit
CCAGGTGACTCTCTTTTCTGTACTTTCTCCTCTTCTAGATTCTGATTCGCAACCGAGTAACTGATCTAGATGCC23940.10787904568952465No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC23910.10774385891547762No Hit
GCCCAAAGATGACAGAGCTGGCAGCAGAGGCTGGCCTCCTGCGGTGCCTC23850.10747348536738358No Hit
GTTTTCTCTGCAGTCTTTAAACCAGAGGAGTTACGCCAGGCCCTCATGCC23760.1070679250452425No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA23730.1069327382711955No Hit
GACCTTCACACGATTCTCCACTTGGTGAAAACATCCCATTGTCATGGTAG23590.10630186665897604No Hit
TTTGTCCTCTTTGTCAAATGTCACGACTTCGGCCACACAGTTAGGGGCAC23510.10594136859485065No Hit
GTCCACAGTGGGGGTCCGGTGCGGCGTTGTGCTCTCTGTAACTGCGGGGA23470.10576111956278796No Hit
GGCATCTAGATCAGTTACTCGGTTGCGAATCAGAATCTAGAAGAGGAGAAAGTACAGAAAAGAGAGTCACCTGG23410.10549074601469391No Hit
GATGTTCATGATATTTTGTTACACGTTCATTGTCAAAACCTTGGTGCAAG23390.10540062149866254No Hit
CGAGACACAAAGCATGGATGAGATGTTGAGTACTGGCAGTCCTCTCCTGT23250.10476974988644311No Hit
CCCCAAATATGGAAAAGCTCTTCCCCCCTCCTTACTCTTTTTTCTAGGCA23230.10467962537041176No Hit
CATCAACACCATCCAACTGTGAGATTTTTGGAAGATCACACTGTTCCTCC23220.10463456311239608No Hit
GCATTTTAAGACTCTTACCATGTCCTTCATCACACAATCTACATCTTCTG23150.10431912730628637No Hit
GAACCAATCAAGCATTGCCCAAGATCGAGGTGAGAGCCTGGGTGTTGGGG23140.10427406504827069No Hit
AGGGCACTCAAATGTCTGCCCTAGCTCCCTGCCCCTCCCCAACACCCAGG23110.10413887827422368No Hit
CCATAACAGCTTTTTTCTCTCCTTGAAGTATTTGTCTGAAAGTGGCCACC23090.10404875375819231No Hit
GAACAGCACCAGCATGTCTAAGGCATATCAGAGCACCTTCACAGGCGAGA23040.10382344246811395No Hit
CTCCCCTACTCGGTGCTGCAGCCTCCGCAAGCCTGTCTTAAACTTGAGTT23020.1037333179520826No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG23010.10368825569406692No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG22990.10359813117803557No Hit
CAAAGCGCCGTAGCTCCCGCTGCCCGTGTAGACTGGGCTCCCCGCAGTTA22990.10359813117803557No Hit
TTTCTACAACCAGCTTGCATTTTTTCTGCCCACAATGAGCGGGGTAAGAT22980.1035530689200199No Hit
CTGTAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAG22980.1035530689200199No Hit
ACTTGAAAAGCAGATTCCTTGGTAAGGATGCCTCCGCCTGAACCGAAGTA22970.10350800666200423No Hit
GAACTTGTATTTCTTCCAGTTGCAGGCTTTGGGGTCAGTGGGGCTCTTGG22820.1028320727917691No Hit
CTGGGTCCTGTCGATACAGTGCTTCTAGGGTTGGCATGAGGGCCTGGCGT22690.10224626343756534No Hit
CTTCCAGGGGTTAATTGTGAAATTAATTTTGATGACTGTGCAAGTAACCC22620.10193082763145561No Hit
GAACCAAGGAACCTGCTTTGATGACATAAGTGGCTACACTTGCCACTGTG22620.10193082763145561No Hit
TGATGTTGTTCTCACAATGGACTCCATCAAATCCTGCCTGGCACTTGCAA22570.10170551634137726No Hit
CCCCAGGAGGCCAAAGACACAGATCAAGGAGTAGGCAATCGGTACAAATA22520.10148020505129887No Hit
CCAGCACTGCCACTGCCTGGAGACACAATGTGGTGGTGGGATAGCAACTG22460.10120983150320483No Hit
CGTTCAGGTGGCCCACCTCCGTGCCCATGTGCTTATCCACACTGGTGAGA22440.10111970698717349No Hit
AAGAATGCCTGCATCCTCCAGGCTTCTGGCTCCCCTCCAGCCAAGAGCCC22430.1010746447291578No Hit
ATGTGCCCTGTGCACCCTCACCTTGTGTCAATGGAGGCACCTGTCGGCAG22420.10102958247114213No Hit
CTGATCTGTATCCTAAATCCTCATAATGGGACCAGAGGATCCCTGTCAAC22410.10098452021312646No Hit
CAGTACCTTTGGTCTAACAGAGGCAGAAGAGTTGGATTCTGTTGAGTTCA22410.10098452021312646No Hit
CCTCTTATGCAGAGCCCCAGTACTTTCGCTTCGAAGGTGTGTGGCTGACA22400.10093945795511079No Hit
GTCCTTTTCCGAAAGGTAGCCAGGGACACTTTTATGCTTTTCAGTCTGCT22360.1007592089230481No Hit
ACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGT22310.10053389763296972No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT22250.10026352408487567No Hit
CAGAACCAATGGAAGTGGATGAAAAGAAACCTGAAGTGAAAGTAGAAGTT22250.10026352408487567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)