FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32899560
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA1172250.356311756145067No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC805690.2448938526837441No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT669000.20334618456903375No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG629100.191218362798773No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC625490.1901210836862256No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG552040.16779555714422928No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT543330.16514810532420493No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC472010.1434700038541549No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA467150.14199278045055921No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT413260.12561262217488622No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC403710.12270984779127744No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC348180.10583120260574913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT83950.032.31963745
GCGTACG23900.028.8674852
CGTACGG24400.028.3067343
GGCGTAC28950.024.533041
CCGCGTT113750.023.9317517
ACCGCGT113650.023.913226
CGCGTTC115600.023.4709138
AGACCGC116050.023.4380994
GACCGCG117650.023.0428565
CCGACTA39000.021.97861118
GCGTTCT125100.021.7785039
ATACGGA126750.021.35293433
TACGGAG127100.021.18792734
CAATACG128150.021.10210231
CGACTAT41200.020.96881719
AGAACGA132700.020.6159144
AATACGG132750.020.40477832
CCCCACT257200.020.2853161
ACGGAGA133900.020.1456135
AAGTCGT43650.019.68878731
Produced by FastQC (version 0.11.5)