FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32899560
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1361820.4139325875482833No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT1073160.32619281230508856No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC792270.24081477077504987No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC609860.18537026027095804No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA568670.17285033599233546No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT554430.16852201062871358No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC410280.12470683498502716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT45250.025.4088291
GGCGATC48950.022.9366252
CGATCTG55700.020.3185714
GGTGGCG91450.019.2154121
GCGATCT59650.019.0862433
TGGCGCG93050.018.3529433
AGGGGAG239000.016.7949711
TCGCACG29450.016.73169127
GGCGCGT102150.016.6959514
CGCACGA29300.016.66376728
CGCGAGT30250.016.58669513
GGTCGAC35050.016.1768841
CGAGTCG31200.016.00958415
TGCGCGA32900.015.38746111
GCGACAT73350.015.09199514
GGGAGAT379450.015.0671961
GCGCGTG117300.014.5779325
TGCGACA76150.014.56657213
CGCGTGC118950.014.3189716
GCGTGCC122450.014.0015647
Produced by FastQC (version 0.11.5)