FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27722708
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG494990.17855037826751988No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT417740.15068513508853465No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC338760.12219585474838894No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC304890.1099784335642824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT43200.026.8855761
GGCGATC44300.026.0655542
GCGATCT55400.021.1998673
CGATCTG59900.019.4946424
GGCGCGT41750.013.2572484
TGGCGCG41750.013.2032853
GGGAGAT195600.012.6353371
GCGACAT91900.012.60842914
TGCGACA92450.012.41173613
CTGCGAC94650.012.14701512
GCTGCGA95750.011.9604711
CGACATC97450.011.86726215
GAGATAC189050.011.7466183
AGGGGGG46000.011.4501551
AGATACC192950.011.3110174
GCCACGA27100.011.29112819
GGTGGCG49750.011.175251
ACGAGCT28800.011.17149422
GCGCGTG50200.011.0705195
AGGGGAG143650.011.01547051
Produced by FastQC (version 0.11.5)