FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28537686
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA841210.29477162233826526No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT765380.26819974121237444No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC576660.2020696422267734No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC540670.18945824829665586No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC498510.17468480100313669No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC481860.16885041064646938No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG461370.1616704311624986No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG367100.12863691891486928No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT352600.12355591830395779No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT351510.12317396722355135No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT340990.11948761367687627No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA320340.11225156797926784No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG303120.1062174417365164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT58800.029.92048545
GCGTACG18250.024.4630872
CGTACGG18450.024.0220363
GATCGGA15400.023.4267081
GGCGTAC21550.021.6588331
ATGGACC201300.019.1895944
CGACTAT28350.018.25214819
CCGACTA28300.018.125418
GTCTGGA238650.017.7721231
TGGACCT219900.017.5562345
TGCCGAC30150.017.53556616
ACGAATC19750.017.42864238
AGAACGA101650.017.32980244
CCCCACT226500.017.2521151
GTACGGA26350.017.0761264
AGTCGTC31000.016.76445832
CAAGTCG31150.016.46705830
GGGGTCA266700.016.30602345
GTCCGTT12050.016.2796171
AATGGAC240450.016.1680743
Produced by FastQC (version 0.11.5)