FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31611194
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC2153760.6813282661831754No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT1838250.5815186860705104No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1665330.5268165447973905No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC1564830.49502400953282566No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC1560060.49351505039638804No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCC1270290.4018481554350652No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA1106960.3501797496165441No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT932690.2950505444368852No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC917290.29017885246599673No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG835170.26420071320305083No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC605470.19153658036453794No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC564580.17860128915092546No Hit
TGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGA401880.1271321798221225No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGA391580.12387384038704771No Hit
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA378350.11968861410296618No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG377470.11941023170462971No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACC352820.11161236111486329No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGA339510.10740182734002392No Hit
GGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCA322620.10205878335377018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC44750.025.843462
AGGGGAG380150.020.7154141
TGCAGGT359950.020.1027013
GGGCGAT63100.019.8612421
CGATCTG60800.019.2433364
GCGATCT65500.018.0342713
AGGTCGA399450.017.8613556
GCTGCAG406900.017.8384571
CTGCAGG409200.017.6392172
GCGCCGA83150.017.2368431
GTTCTAT125450.016.859231
GCGACAT70950.016.2367414
GGTCGAC613850.016.1899591
TGCGACA71750.016.08703613
TGGCGCG116350.015.6251993
GGGAGAT647700.015.2014141
CGACATC77800.014.80715615
CTGCGAC78700.014.666388512
ACGGGCT42150.014.57288845
GCTGCGA80800.014.36874911
Produced by FastQC (version 0.11.5)