FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38651155
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT1281290.331501089682831No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1208050.31255210872741057No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC1093910.28302129651752966No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT853360.22078512272142967No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC755480.19546117056527806No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA650020.16817608684656385No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC541440.14008378274853625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGCA77350.021.6122363
CTTCCGC80000.020.7614862
TCCGCAG80650.020.7280794
AGGGGAG270150.020.3754181
CCTTCCG89900.018.6216051
CGCAGGT95800.017.567286
GGTGGCG132850.016.6208231
TGGCGCG135800.015.7893543
GGCGATC35300.015.7475962
GGGCGAT39800.015.2851131
TACGCTA16050.014.9994759
CGGTGGC109750.014.8840631
TATACGC16450.014.7715197
GGGGAGA376600.014.742832
GGGAGAT469400.014.5921231
GGTCGAC77000.014.0748031
GTGGCGC154400.014.0368942
CGATCTG41200.013.9803284
GCGATCT43200.013.437153
GGCGCGT159250.013.4361754
Produced by FastQC (version 0.11.5)