FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31876973
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2067630.6486280864873839No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT330410.10365162338343732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC55050.018.6374911
AGGTCGA39550.014.62064556
GTCGACC70400.014.5418182
TCGACCC73000.014.1163543
CCGTGCG77050.013.4619588
CGACCCG77850.013.3236214
TGCGATT28900.013.3130935
CGTGCGG78750.013.1427789
GCCGGGA80550.013.12847543
AGGGGGG138100.012.8058611
ACCCGTG82550.012.6468066
GCGATTT31400.012.61140936
CTGCGAT30400.012.58220234
CGGGACC83850.012.53128945
CCGGGAC83800.012.48506744
TCGCATA8150.012.423248
GACGCGC83350.012.30950432
CGCGCGA83900.012.22880934
GTGCGGA85400.012.22475210
TGCGGAG88450.011.93039711
Produced by FastQC (version 0.11.5)