FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27716997
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA1992930.7190281111622591No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG1205340.43487395117155003No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1070960.3863910653812893No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC1037410.37428657945880645No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA885100.31933473889685815No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC831270.29991344300394446No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTG751790.27123789781411023No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT747250.2695999137280276No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG680470.24550639450586945No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC648320.23390701380816975No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG621450.22421260138679525No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTC604780.21819824131741253No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT576440.20797346841001568No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCT566050.20422486606323187No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA509510.1838258307709165No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA480950.17352168418533942No Hit
CTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGG470940.1699101818281396No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC466180.16819282406387676No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGC447770.1615506903579778No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGG433980.1565754038938634No Hit
CCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAG431210.15557601712768523No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGG402630.14526465475318268No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCT395480.14268501021232569No Hit
CCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATA376950.13599958177287388No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT349080.12594437990522567No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT341110.12306888801842421No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCC334180.12056861715574743No Hit
GTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGG330770.1193383251439541No Hit
CCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATAA329230.11878270939669258No Hit
CACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGG325020.11726378582788026No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCC314210.11336365191366149No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCT303110.10935888906002335No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCC295630.10666018400189602No Hit
CCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGT282690.10199156856711424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAC92700.029.0751551
GCGTACG94850.028.29722
CGTACGG102900.026.2359983
CCCCACT254900.024.9878391
CGCTGAG41900.024.37675544
TCCGAGA61550.023.53903841
TTAACGA75750.022.98786517
GAGCGCT44950.022.972841
CCGCGTT36050.022.7157887
ACCGCGT36000.022.6848456
TAACGAG76850.022.54172718
CACCGTT31200.022.3376621
GTCCGTT9600.022.084651
TGATCGT105300.021.8564174
AGACCGC37500.021.8374444
TTTAACG80050.021.83688416
AGCGCTG48000.021.79428742
CGAGGAT79650.021.72105421
GATCGTT106650.021.6430385
CGAGATC67700.021.53378543
Produced by FastQC (version 0.11.5)