FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27716997
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC1425120.5141682556735855No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT1419190.5120287742571824No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1318790.4758055138513021No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC1125970.406238092820806No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT942650.34009817152991No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC675760.24380707621392028No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA637210.22989864306006885No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT636380.22959918781966174No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTA528930.19083236181755187No Hit
TGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGA500410.18054264680982576No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC474250.1711043948953056No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCC432330.15598010130751178No Hit
GTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGG430710.15539562240454838No Hit
CATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCG429370.1549121645465416No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGA414100.1494029097019421No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC405580.14632898361969013No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG339510.1224916249043863No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACC335720.12112423290300894No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCA328000.11833893837777591No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGA300050.10825487335442581No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAA284200.10253636063098755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGCA108850.025.755113
TCGCATA13800.025.434328
AGGGGAG232800.024.681191
CTTCCGC113550.024.635272
TCCGCAG114550.024.4933574
TACGCTA53900.021.3725249
TATACGC53800.021.370087
CGCAGGT133900.021.0708926
ACGCTAT55050.021.00779210
ATATACG55750.020.783966
ATACGCT57250.020.2791068
ATCGCAT18200.019.4086727
CGCATAA18500.019.2158669
CGCTATT60750.019.07365811
CCTTCCG151550.018.7485661
GGTGGCG83250.016.9162731
AGGTTCA169400.016.854779
GTTGTCG20350.016.80507526
GGGAGAT450100.016.6352841
GGGGAGA348400.016.633072
Produced by FastQC (version 0.11.5)