FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27060467
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC611540.22599018708731078No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG595690.22013293414337604No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT505490.18680017606495852No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA477890.17660079554428976No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT432290.15974964511883702No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA423760.15659744526951438No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTC375160.13863766652659765No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG358940.1326436827568423No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT330470.12212280002410897No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT323250.11945470120674562No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTC318500.11769937303742764No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTC314240.11612512082662875No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC310300.11466912230302603No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGA296110.10942531036142132No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT273870.10120667910128824No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATC272060.10053780668308496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC55550.027.1041032
CGGTGGC176650.026.4294911
GGGCGAT60650.025.6348611
GCGATCT68200.022.136963
CGATCTG79750.019.1852254
GGTGGCG277150.016.695572
TGGCGCG290950.015.6987964
GGCGCAA65500.014.873569545
GGCGCGT318350.014.0719774
TGGCGCA71550.013.67883344
GCGCGTG335900.013.403885
GTGGCGC356800.012.8138223
CGCGTGC361500.012.4415566
TGCGACA123050.012.30560213
GCGACAT122850.012.2519714
GCGTGCC371450.012.205387
GGTCGAC19250.011.8223371
CTGCGAC129450.011.61031112
GCTGCGA130600.011.54263711
GCCACGA58550.011.52798419
Produced by FastQC (version 0.11.5)