FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25648769
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC981680.3827396160806002No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC719460.28050469010812956No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT604080.23552007505701347No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG545240.2125794029335287No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC537180.2094369519254511No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC475860.1855293717994809No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA475500.1853890141862169No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGT450920.17580570825835737No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT449770.17535734366043063No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCT398360.1553134967218115No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG396960.1547676615591181No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA352750.1375309668857792No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT334500.13041561565781187No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGC304590.11875423728912682No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA292380.11399377490592238No Hit
CTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCACGTGT276130.10765818819608848No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC272270.106153242676091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAC71200.025.8607081
GCGTACG71450.025.5491942
CGTACGG75750.024.1479023
CCCCACT239850.020.0085031
GATCGTT43100.019.523795
TGGCGTA34150.018.850982
TGATCGT45850.018.5981454
GAGCGCT30950.018.39253241
GTCGGAT14450.018.3815141
GTACGGA100500.018.2457334
TAACGAG24050.018.0557818
GTCCGTT12450.017.718371
TACGGAA104250.017.6109945
TTAACGA25200.017.49965717
TTTAACG25050.017.42467316
CGCTGAG34450.016.98109844
TGGCGAC19500.016.8529852
TCGCGCG31850.016.67186545
AGCGCTG35350.016.61241342
GTTCCGT12900.016.576811
Produced by FastQC (version 0.11.5)