FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38791434
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1534120.3954790637541268No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAA803880.20723131813069864No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGA689730.17780471843345622No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC671280.17304851375177313No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGG625020.1611232005498946No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACC562730.14506553173569195No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG527150.13589340368288524No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT499130.12867015949964625No Hit
CCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCG491690.12675221029467484No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG466040.12013992573721302No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT461610.1189979210358658No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC437400.11275685245355972No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGAT429330.11067649625945769No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAG409090.10545884949754629No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC408280.10525004051152118No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACTC393650.1014785893194874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC67200.023.4763892
CGGTGGC128400.020.987881
GGGCGAT81300.020.4812451
TGGCGCA199950.019.02778844
GGCGCAA202300.018.82899545
GTCTAAC209850.018.7365191
GGTGGCG212650.018.3626611
GCGATCT87600.018.3389153
ACGTGCG218650.017.6268658
CGCGAGT218450.017.59132413
TGCGCGA220150.017.47603411
GCGAGTC225800.017.06839814
CGAGTCG225000.017.05906915
TAACACG228050.016.9403344
GTGCGCG233350.016.56466110
CGTGCGC234450.016.5157329
AACACGT236500.016.4873075
CGCACGA234050.016.38051628
TGGCGCG234800.016.3095133
ACACGTG240550.016.1437246
Produced by FastQC (version 0.11.5)