FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004966768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31508409
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGA3728721.18340472221241No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3613261.1467605362111428No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT3312531.0513161740410315No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACC3153231.0007582420299292No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC2773230.8801555165797168No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC1943570.6168416818507084No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGG1942810.6166004763998081No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA1913550.6073140665401418No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT1744080.55352842474528No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT1679870.5331497379001269No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA1663930.5280907709430839No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT1636290.5193185095445473No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCAC1540860.4890313566768795No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG1339130.42500717824248124No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTC1295420.4111346910597739No Hit
GCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGG1129080.3583424348719099No Hit
GATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC1075870.3414548795529473No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACTC1070500.33975057261697983No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGA1007590.31978447404310384No Hit
GTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCC996160.3161568710117988No Hit
ACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG937910.2976697427026544No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT914880.2903605827891849No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC910520.2889768252024404No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG901690.28617439871368944No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCG870300.2762119788403153No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG869630.275999337192811No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATC852900.2706896435170687No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATC839100.26630986032966625No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT834750.26492927649885467No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT832890.2643389578953352No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAG818020.25961958282311237No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT771370.24481401139613235No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC767740.24366193799248956No Hit
GGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAAC758290.24066273863589874No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT687700.21825919550555536No Hit
TGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGA620980.19708389592124437No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGC594580.18870518025838753No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGC583270.18511566229827725No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT556340.17656873757097669No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAA549990.17455340255358498No Hit
GGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATC539710.17129078145456345No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT531870.1688025568031696No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC530000.16820906444371722No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC523400.166114385528003No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACC509100.16157591454395556No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGG507640.16111254617775211No Hit
GTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG491910.1561202280952999No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC480270.15242597618940393No Hit
GGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTA478160.15175631368756196No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA469630.14904909987679796No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATC465820.14783989886636295No Hit
GCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTC464460.1474082680594885No Hit
GGACGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGA451000.14313639257380467No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA450580.14300309482462284No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCA449790.14275236810592373No Hit
GGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC447070.14188910649217484No Hit
GATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTA441310.14006102307482424No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCT431140.13683331329106463No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGT408170.12954319591319258No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG405820.128797363268961No Hit
CCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCC404290.12831177861122722No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC395460.12550935212247627No Hit
GGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCAT387570.1230052586914179No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAG386820.12276722699645039No Hit
GAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTG380930.12089788475197209No Hit
AATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGG373370.11849852526669945No Hit
CCTGTATCCTAGGCTACACACTGAGGACTCTGTTCCTCCCCTTTCCGCCTA371280.11783521027672328No Hit
GATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCA355410.11279845961121046No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCG348450.11058952548191182No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT346070.10983417156988157No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC340860.1081806447288405No Hit
GATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGAT339940.10788865918301366No Hit
GGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG334810.10626052238943579No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTC334060.10602249069446826No Hit
GGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACTCC334040.10601614318260245No Hit
GTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACC334000.10600344815887086No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTC333990.10600027440293795No Hit
ATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTAT332260.1054512146265462No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG329650.10462286432805921No Hit
TGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAA322770.10243932024622379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)