FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30665423
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC643910.2099791677421179No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA619130.20189840524945635No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT496850.16202287507985785No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT488320.1592412405333525No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC486760.1587325242505215No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC464560.15149310022561893No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG457780.14928214099639192No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCT431330.1406567912009562No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC370740.12089838121587301No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA346400.11296110280298433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA6700.029.21346543
GGCGTAC69600.028.8394581
CGCGTAT6800.028.78385244
GCGTACG72850.027.3980772
TCGTATG25650.026.7516845
ATCGCGT7550.026.51997642
CGTACGG76150.026.323323
TCTCGTA27700.024.77186243
ATCTCGT30300.023.01702142
GTACGGA99300.020.277144
TACGGAA106750.019.0306135
GGTCGAC67100.018.5869851
CCGCTCG111450.018.06672528
ACCGCGT36800.017.973496
CGTCTGA49350.017.96135716
TGGCGTA29850.017.9064872
CGCTCGT112200.017.90585529
AGACCCG113500.017.83925611
ACCCGCT113900.017.79635813
CGCGTTC37950.017.3101628
Produced by FastQC (version 0.11.5)