FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30667850
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1869170.6094884382178731No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT704270.22964439959110272No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT679870.22168818485808428No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC677950.22106212205942055No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACTC601770.19622177622493914No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTC498740.16262633344039443No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT498620.16258720451547795No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC483090.15752326948253628No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATC456340.14880077996990332No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC420880.13723818265708226No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGAT406410.13251988646090287No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA406340.1324970612547016No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT403410.13154166333799075No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACC393500.12831026628863776No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT374470.12210507094563199No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT355190.11581835700905019No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG332660.10847190135598028No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC329620.10748063525809604No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCT328140.10699804518412605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC112450.029.5033041
GGCGATC62700.024.984712
GGTGGCG222400.022.1940461
TCAGACG49900.021.831192
GCGATCT73500.021.5504553
GGGCGAT80300.020.5457041
ACGTGCG76350.019.0072368
TGCGCGA76900.018.84206811
CGCGAGT77050.018.68858113
CGAGTCG77900.018.4557815
GGCGCAA78550.018.3893245
TGGCGCA80000.018.14038744
GGGAAGC162950.018.0946241
GCGAGTC80250.018.02747714
TGGCGCG274700.017.9290893
CGTGCGC81150.017.9106889
GTGCGCG81850.017.70253810
GTCTAAC88500.017.6755981
GCCACGA159850.017.63618519
CGCACGA81950.017.626628
Produced by FastQC (version 0.11.5)