FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45055504
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1363070.3025312956215072No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTC1026290.22778349122451277No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT976790.21679704215493847No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC855650.18991020497739855No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGAT741390.1645503732462964No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA680960.1511380274427737No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATC676260.15009486965232927No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG667230.14809067500387965No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTC639790.14200040909541262No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT603260.13389263163053286No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT596540.13240113793866337No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT571580.12686130422600533No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC546550.12130593412072363No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT541710.12023170354503192No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT515840.11448989672826652No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC512020.11364205358794788No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGC475660.10557200736229695No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACC452100.10034290150211171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC197050.030.118261
GGCGATC71500.023.9532592
GGGCGAT75900.022.9471231
GGTGGCG350200.021.5020981
TCAGACG62700.020.3517382
GCGATCT90000.019.7748363
ACGTGCG98400.019.755438
TGCGCGA99350.019.47587611
CGCGAGT99800.019.36542713
CGAGTCG101450.019.0502115
CGCACGA104850.018.28250128
GTGCGCG107350.018.21317110
GCGAGTC107000.018.12527514
GGCGCAA106300.018.09665545
TAACACG109100.018.0453834
CGTGCGC109500.017.8350149
TGGCGCA108600.017.79628244
GCTCGCA110000.017.54934925
CTCGCAC111450.017.34117726
GTCTAAC117600.017.3393081
Produced by FastQC (version 0.11.5)