FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38555486
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1263740.32777177286780923No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA1215240.31519249945390393No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG1156140.2998639415412893No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT1081570.2805229844593322No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1068130.2770370992081386No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT976020.2531468543802041No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG906510.23511829159668743No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC902170.23399264115098953No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA650400.1686919469774029No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA640320.1660775330390077No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA570080.14785963273812708No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT525990.13642416542226962No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC512900.13302905843282586No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG509430.13212905680919182No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC490730.12727890396712935No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT473740.12287226777533033No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT454750.11794689865924657No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG405970.10529500263594135No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT401470.10412785355630065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT77250.026.99744245
GGACCTT231000.023.79320144
ACCGCGT97300.021.5263486
GACCTTG257100.021.4127945
CGACTAT52800.021.30507719
CCGCGTT98850.021.143157
CCGACTA53950.021.01774418
GACCGCG101650.020.5608865
AGACCGC103100.020.4462874
AGTCGTC56200.020.05533232
CGCGTTC107950.019.3815618
GCCGACT59300.019.31123717
CGTACGG11800.019.25633
AGAACGA110300.019.1731644
TGCCGAC59100.019.10929516
CAAGTCG59000.019.06574230
CCCCACT284000.018.9642221
AAGTCGT59600.018.91155231
TCCGAGA72000.018.77893441
GTCGTCA60750.018.62730833
Produced by FastQC (version 0.11.5)