FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38555486
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1547930.40148112774405176No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT968390.2511678882740578No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC874010.22668888157706016No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC859040.22280616563878872No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC769820.19966548988644572No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT575900.1493691455477957No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA526330.13651235001939802No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG385770.1000558001006653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC55900.024.5581682
GCGATCT65550.021.006633
GGGCGAT67500.020.9281561
CGGTGGC115450.018.5589681
CGATCTG78300.017.9022164
TCGCATA17400.016.8098748
GCCACGA109700.016.51034719
GTTGTCG32050.016.00558526
TGGCGCG169700.015.7246323
GGTGGCG173600.015.716711
ACGAGCT117250.015.38964122
GTCGTTC33300.015.06687229
GGCGCGT181950.014.6536834
AGGGGAG298000.014.1910721
GAGTGCG127600.014.15882929
AGTGCGT129600.013.92282430
CACGAGC130150.013.84698821
CGCATAA21900.013.66400059
GCGCGTG200850.013.3420185
CGAGCTG138550.013.2185323
Produced by FastQC (version 0.11.5)