FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25871620
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1873720.7242376008924065No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT1102850.4262779060607724No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC665440.2572084778610694No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC655730.2534553305900442No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT562570.2174467621277678No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC555630.21476428611737494No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA518080.2002503128911139No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACC507960.1963386908125583No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGA429850.1661473073584105No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC424550.16409873057813928No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC399050.15424237059758916No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCC392740.15180340465730402No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGA309510.11963301872863007No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTC301080.11637462207623643No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG299250.11566728330116166No Hit
TGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGA296410.11456955536607294No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA292680.11312782114146697No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGG286390.11069658567959796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC25150.021.0237432
AGGTCGA106100.019.1916966
TGGCGCG96200.019.0617473
GGGCGAT31350.018.8754961
GGTGGCG110050.017.5419311
AGGGGAG197900.017.2472531
GGCGCGT109600.016.7722634
GGGAGAT346800.016.6802731
GGTCGAC158900.016.6802121
CGATCTG32150.016.3062884
TGCGCGA48950.015.12249211
ACGTGCG48550.015.061718
CGCGAGT48850.015.06133113
CGCGTGC123150.014.9268376
GCGATCT35350.014.8301883
CGAGTCG49850.014.80441915
GCGCGTG125200.014.7902565
GCGACAT33700.014.55490214
TGCAGGT144000.014.4530483
TGGCGCA49450.014.42361144
Produced by FastQC (version 0.11.5)