FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28657713
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT930130.32456532731694254No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG852310.2974103341742588No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC838040.2924308719261722No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG715320.249608194485024No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC706470.24652002063109504No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC651690.22740474789457202No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT641800.22395366999453167No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA639540.2231650515866357No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT624880.2180495003212573No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA605740.21137067008801433No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA493070.17205490193861597No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG464230.16199129358298758No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC357610.12478664993260279No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC324750.11332027786027449No Hit
CTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCACGTGT313060.10924109680350279No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT308990.10782088577689364No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT308580.10767781783563818No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGA306790.1070532041408887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT45150.030.54524445
ACCGCGT55050.025.2968166
CCGCGTT55550.024.9879917
GACCGCG56000.024.7873275
AGACCGC56500.024.6874264
AGAACGA58750.023.51263844
CGCGTTC59300.023.3698718
CAGACCG61300.023.2314223
GGACCTT191500.022.67406744
ATACGGA62450.022.2991633
TACGGAG63150.022.0519834
CAATACG64000.021.7593331
AATACGG66800.020.84704432
GCGTTCT66750.020.7952529
GACCTTG212250.020.64819745
AAGAACG66950.020.63282443
GTCCGTT11600.019.8314461
ACGGAGA71900.019.52477335
ATGGACC231000.018.67957142
CGGAGAG77100.018.41218436
Produced by FastQC (version 0.11.5)