FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34511764
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT1599980.4636042365148301No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC1176200.3408113245095209No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC1112820.32244657213117245No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA835180.24199864139080227No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG827190.23968348879529888No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT803140.23271485050720678No Hit
TGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGA527950.1529768226277857No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC466240.13509596321996173No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC432740.12538912818249454No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC401410.11631106425043936No Hit
CATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCG368550.10678967322562824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT69450.030.4858841
GGCGATC77300.026.8951442
GCGATCT95700.021.9356173
CGATCTG99800.021.1020894
AGGGGAG305900.019.2856661
TCGCATA15300.017.794028
TGCGCGA45550.016.20188711
ACGTGCG44900.015.9854368
GTTGTCG35200.015.98007326
GGGAGAT535050.015.8746441
CGCGAGT45850.015.85051413
CGCATAA16750.015.5820059
GGCGCAA45550.015.510365545
GTCGTTC36000.015.43743929
CGCACGA46350.015.29117928
GGTGGCG95350.015.1258131
TGGCGCG92800.015.0322473
CGAGTCG48650.014.98456615
TCGCACG47200.014.92051327
GGGGAGA403300.014.7340332
Produced by FastQC (version 0.11.5)