FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004966790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29442330
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT1415920.4809130255655717No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1202240.4083372477653773No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC960090.32609171896381844No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA764400.2596261912695089No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT724920.24621692644569912No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC712390.24196114913459635No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC401110.13623582101008988No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC372820.12662720647448758No Hit
TGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGA347970.11818697772900447No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT299560.10174466490933291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT26500.024.367861
AGGGGAG221700.022.6623231
TATACGC23150.020.5074377
GCGGCAT54450.020.45452929
TACGCTA23700.019.936599
AACGCTG61650.019.85426722
ACGCTGG66400.018.53562723
CGCTGGC66050.018.39545224
ATATACG26300.018.0512546
ATACGCT26950.017.8663148
GCTGGCG70100.017.52524625
GGTGGCG50500.017.3316381
GGTCGAC108050.017.3252681
TTCCGCA54400.017.1644483
TGGCGGC71200.017.0962527
CTGGCGG72250.016.9723626
ACGCTAT28700.016.93369710
GGGAGAT461550.016.9304081
GGCGGCA69450.016.91143228
CGATCTG36900.016.8901714
Produced by FastQC (version 0.11.5)