FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004966791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32970872
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA3708781.1248656086499622No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCT3242630.9834832393877845No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG2081400.6312844865006907No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC2052530.6225282728342763No Hit
GTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGG1856700.5631334227374999No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCC1819410.5518234397925539No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGT1695450.51422661796752No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC1603250.4862625410695841No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1536560.46603559651076265No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC1534130.4652985823365545No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTG1391590.4220664834099626No Hit
CCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGC1329640.40327717143786795No Hit
GCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGG1308090.39674109923450007No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC1304530.39566135830438454No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCT1010490.30647961024506726No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA970990.2944993386890101No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG907500.2752429477752363No Hit
CCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGG899200.2727255742583939No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC889590.2698108803431101No Hit
CCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATA866940.2629411803242571No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA743800.22559306287076664No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC742350.22515328075035443No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGG719880.21833817437403535No Hit
CGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCC707760.21466220244341733No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT706450.21426488204497593No Hit
GGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCG693560.2103553706435183No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT682230.20691900414402142No Hit
CCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGC654570.19852978107464067No Hit
CCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCC640570.19428360887755713No Hit
GCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGC639570.19398031086347975No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT620610.18822978051657233No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG605850.18375310182878998No Hit
GCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTT599520.18183322539968005No Hit
GCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGC595540.18062609930365203No Hit
GCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCGT581120.17625254194065598No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC559140.16958605159123485No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG559120.16957998563095328No Hit
CCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACC540510.16393560958897296No Hit
GTGGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTC523150.15867035606458937No Hit
GTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCC503190.15261652770360457No Hit
GCCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC501150.15199779975488667No Hit
CCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATAA487170.14775769351808468No Hit
GCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGT486650.14759997855076443No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC468080.1419677344293472No Hit
GCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGC467370.14175239283935226No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA466950.14162500767343975No Hit
GTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTATT449130.13622023706258057No Hit
GTCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGG448410.13600186249244486No Hit
GGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCG448210.13594120288962938No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGA414060.1255835757088863No Hit
GCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCAC406340.12324211504020882No Hit
GGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCC391920.11886855767721277No Hit
GCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCC390940.11857132562341695No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCC387690.1175856070776654No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCA376450.11417653739943547No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT363200.11015783871290998No Hit
ACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTG361570.10966346294996383No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA356790.1082136984426739No Hit
CCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGG352220.10682762651834019No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG346820.10518981724232225No Hit
GGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCG341310.10351864518475581No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT340390.10323961101180461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)