FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004966792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004966792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32970872
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC5355221.6242275909475492No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC5080831.5410056488648527No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT3334911.0114715801268463No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCC3287490.9970891882992964No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG2224510.6746894652953067No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC2070640.6280209998692179No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA1769870.5367980561751597No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1657880.5028317115786322No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT1563420.47418218116888144No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC1547310.4692960501620946No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG976970.2963130608131929No Hit
TGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGA939690.2850061108483877No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC899130.27270434339740846No Hit
GGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC799240.24240790477121746No Hit
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCT759920.23048222685769426No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGA722960.21927233225739373No Hit
GTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGG720350.21848072444065175No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC666970.20229067644920037No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCG635310.19268826132351002No Hit
GATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTA624220.18932468634739172No Hit
GCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCG587550.17820274817117362No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGT583810.17706841359852418No Hit
GCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGG546610.16578572747484507No Hit
CCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGC541720.1643026001860066No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG523350.15873101566740486No Hit
CATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCG499110.1513790718061688No Hit
GAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTG488250.1480852553732883No Hit
AAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTC464940.1410153786651442No Hit
ATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATA460700.13972939508545604No Hit
ATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTAT456840.13855866475111728No Hit
GATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCA435820.13218334049521044No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGA420180.12743975955503997No Hit
CTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCC416910.1264479750490069No Hit
CCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCT410710.12456752736172705No Hit
GCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG388580.11785554231019428No Hit
AGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACC385070.11679096628078262No Hit
GTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATATATT367810.11155604255780678No Hit
GATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGAT365050.11071894003895316No Hit
TGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGG356730.10819550056182924No Hit
GGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCC355880.10793769724986345No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTC355250.1077466195009947No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACC345810.10488348624810408No Hit
GGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCA333660.10119841537706373No Hit
ATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGG330570.10026122451356459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)