FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004968268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004968268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20168573
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7177293.558650381462288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1062750.065.3280812
ATCAACG1053950.065.314211
CAACGCA1065200.065.1707713
AACGCAG1069750.064.6794514
ACGCAGA1073950.064.1678615
GGTATCA1078350.063.9564828
GTGGTAT1084050.063.6670726
GTATCAA1084550.063.490619
CGCAGAG1086450.063.4490916
GAGTACT851650.063.1762520
TGGTATC1091600.063.1497277
AGTGGTA1094150.063.1128435
TATCAAC1090950.063.0926210
AGAGTAC1079950.062.96626719
AAGCAGT1134950.061.4422451
CAGAGTA1124400.060.883818
AGTACTT888600.060.7055521
GTACTTT890550.060.66788522
GCAGAGT1137100.060.5483417
AGCAGTG1169900.059.5643542
Produced by FastQC (version 0.11.5)