FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004968269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004968269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21159914
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1971660.93179017646291No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT415500.19636185666917172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA142900.023.210313
TCAACGC148350.022.47540912
ATCAACG149500.022.37429211
AACGCAG161300.020.62845814
GGTATCA164850.020.3326458
ACGCAGA166250.019.91846315
GTGGTAT169800.019.8843736
GAGTACT155900.019.62946520
GTATCAA174700.019.2854549
AGTGGTA175850.019.1812045
CGCAGAG178650.018.71209116
TGGTATC182500.018.4426577
AAGCAGT195550.017.985751
TATCAAC189650.017.87601710
AGAGTAC185200.017.8269319
GTACGGG42850.017.5478521
GTACTTT194150.016.09861622
AGTACGG47600.015.94407720
AGTACTT195900.015.87097521
CAGAGTA218200.015.40327718
Produced by FastQC (version 0.11.5)