FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004968270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004968270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21159914
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8837574.17656234330631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA1288250.065.8635613
TCAACGC1292900.065.6650412
ATCAACG1282000.065.5600311
AACGCAG1300050.065.12250514
GGTATCA1300550.064.661568
ACGCAGA1304050.064.6529215
GTGGTAT1309500.064.2819756
GTATCAA1310150.064.1759
CGCAGAG1314650.064.1202216
GAGTACT1042850.064.0883820
TATCAAC1312800.064.0459110
AGTGGTA1314750.064.0189065
AGAGTAC1301450.064.0066919
TGGTATC1313450.063.9953467
AAGCAGT1353050.062.7838551
CAGAGTA1347100.062.26204318
AGTACTT1080150.061.95619621
GTACTTT1084800.061.88641722
GCAGAGT1368000.061.56628817
AGCAGTG1394800.060.9243622
Produced by FastQC (version 0.11.5)