FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004968272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004968272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17406585
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2841521.6324396772830512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA462200.060.4832613
ATCAACG458900.060.20271311
TCAACGC464300.060.17253512
AACGCAG472350.059.09306714
ACGCAGA475100.058.55472615
GGTATCA472850.058.5303088
GTGGTAT481400.057.6785776
GTATCAA482950.057.291589
GAGTACT384300.057.1687920
AGTGGTA487050.057.0519685
CGCAGAG487550.056.99465616
TGGTATC486350.056.9547237
AGAGTAC483900.056.71614519
TATCAAC490200.056.46584310
AAGCAGT524600.053.9757841
CAGAGTA515750.053.7963618
GTACTTT410500.053.74735622
AGTACTT412350.053.35310421
GCAGAGT529800.052.49517417
AGCAGTG552450.051.1339232
Produced by FastQC (version 0.11.5)