FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004968274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004968274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17896557
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1922471.0742122074095033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC349650.057.38773712
CAACGCA349950.057.3480613
ATCAACG347200.057.2586711
AACGCAG358100.055.8843514
ACGCAGA361250.055.15315
GGTATCA363050.054.970628
GTATCAA370450.053.787779
CGCAGAG373150.053.45046216
GTGGTAT375400.053.3290526
TATCAAC373150.053.3236510
AGAGTAC371500.053.2729919
GAGTACT281600.053.10201320
TGGTATC383200.052.1066137
AGTGGTA387400.051.6582035
CAGAGTA397150.050.17534318
AAGCAGT412250.049.426791
GTACTTT309550.048.79097722
GCAGAGT416350.047.98729317
AGTACTT313400.047.8962821
AGCAGTG443800.045.786152
Produced by FastQC (version 0.11.5)