FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004968277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004968277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21090754
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970120.45997407205071944No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT321890.15262138091412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA121850.023.3230513
TCAACGC124500.022.82656312
ATCAACG126150.022.41832511
AACGCAG138500.020.51986314
GGTATCA142600.019.8554468
GTGGTAT145050.019.6895126
ACGCAGA145450.019.57221815
GAGTACT137700.019.1793620
GTACGGG39050.018.36317421
GTATCAA156850.018.1625549
TATCAAC158400.018.1615410
TGGTATC158900.017.9065787
AGAGTAC158950.017.84770819
CGCAGAG162950.017.59903516
AGTGGTA163850.017.4738125
AAGCAGT184750.016.3333951
GTACTTT166700.016.1106422
AGTACGG45750.015.97998120
AGTACTT169500.015.76661921
CAGAGTA189100.015.22405718
Produced by FastQC (version 0.11.5)