FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004968418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004968418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387871
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279752.015677249542645No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221511.5960417070462602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220821.5910700634280854No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85740.6177807591627752No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34810.25081581789662005No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32880.23690962632694246No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC31800.22912792327240789No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31490.2268942862845322No Hit
TCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTAGG26100.1880578238179197No Hit
ATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCGATGT25190.18150101846641364No Hit
GTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAA23180.16701840444825206No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22560.16255113047250067No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21930.15801180369068882No Hit
ATCAATAACCTACAAAAATGTCGCGCGCATCACAAAGACATCGACGCATTT20620.14857288609676259No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGA17420.12551598815740078TruSeq Adapter, Index 12 (96% over 29bp)
CATCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTA16650.11996792209074186No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)