FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004968427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004968427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016354
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251872.4781719755124687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184391.8142300812512175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183491.805374898903335No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82910.815759076069952No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34030.3348242836649435No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30380.29891159969853026No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29680.292024235650177No Hit
TCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTAGG27020.2658522522664347No Hit
ATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCGATGT25860.25443890612916364No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25640.2522743059996812No Hit
ATCAATAACCTACAAAAATGTCGCGCGCATCACAAAGACATCGACGCATTT22690.22324898608162116No Hit
GTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAA22560.221969904186927No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.2165584038632209No Hit
CATCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTA18260.17966181074704288No Hit
ACGCAGAGTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGT13540.1332212988781468No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.12289025280561694Clontech SMART CDS Primer II A (100% over 21bp)
GTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCG12440.12239829823073457No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10670.10498310627989854No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)