FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004968430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004968430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025756
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280112.7307663810886798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218152.126724094229037No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217082.1162927635812028No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91690.8938772963550785No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36170.352617971525392No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35130.3424791080919829No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31900.3109901380055296No Hit
ATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCGATGT28070.273651823630571No Hit
TCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTAGG27950.27248195477286996No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26880.2620506241250356No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24650.24031056118609104No Hit
GTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAA24300.23689844368446297No Hit
ATCAATAACCTACAAAAATGTCGCGCGCATCACAAAGACATCGACGCATTT23820.23221896825365876No Hit
CATCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTA19770.19273589430624827No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC13910.1356072984218469No Hit
ACGCAGAGTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGT13470.13131777927694308No Hit
GTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCG12500.12186133934385956No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.12078895955763358Clontech SMART CDS Primer II A (100% over 21bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12170.11864419998518166No Hit
CTACAACACCGTCCTCCCACGTGCCCGGGTCCGTGTTGCTTGGTCTTCGTC11950.11649944041272974No Hit
GGTATCAACGCAGAGTACATGGGTTTATCAATAACCTACAAAAATGTCGCG11410.111235030553075No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10260.10002378733343992No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)