FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004968432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004968432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237161
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295162.3857848735936553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283722.2933150980349364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238181.9252142607146523No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106430.8602760675449678No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC86240.6970798465195718RNA PCR Primer, Index 39 (95% over 21bp)
GTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAA68980.5575668809475889No Hit
TCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTAGG68560.5541720115651884No Hit
ATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCGATGT65860.5323478512497565No Hit
ATCAATAACCTACAAAAATGTCGCGCGCATCACAAAGACATCGACGCATTT49090.39679556662390747No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46520.376022199212552No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA45610.3686666488840175RNA PCR Primer, Index 39 (96% over 30bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42720.3453067143241664No Hit
CATCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTA42270.3416693542715944No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40370.3263116118274016No Hit
ACGCAGAGTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGT25550.20652122076269785No Hit
CTACAACACCGTCCTCCCACGTGCCCGGGTCCGTGTTGCTTGGTCTTCGTC24670.19940816110433482No Hit
GTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCG23530.19019351563781917No Hit
TCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCGATGTC22720.1836462675431896No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC22610.18275713508589425RNA PCR Primer, Index 39 (95% over 24bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21200.17136007358783537No Hit
GACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTAGGTTATTGA19500.15761893561145235No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19160.15487070801617575No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG18980.15341576399514695RNA PCR Primer, Index 39 (96% over 26bp)
GGTATCAACGCAGAGTACATGGGTTTATCAATAACCTACAAAAATGTCGCG18360.14840429014493667No Hit
GAGTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGT18130.14654519500695545No Hit
TATCAACGCAGAGTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGA17390.1405637584760593No Hit
CAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTAGGTTA16750.13539062417906803No Hit
CCTACAAAAATGTCGCGCGCATCACAAAGACATCGACGCATTTACCGAACA16420.13272322680718193No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTT16390.13248073613701045RNA PCR Primer, Index 39 (96% over 29bp)
GTATCAACGCAGAGTACATGGGTTTATCAATAACCTACAAAAATGTCGCGG15330.12391273245761869No Hit
ATCTACAACACCGTCCTCCCACGTGCCCGGGTCCGTGTTGCTTGGTCTTCG15210.12294276977693283No Hit
ACCTACAAAAATGTCGCGCGCATCACAAAGACATCGACGCATTTACCGAAC14950.12084118396878014No Hit
GAGTTGTTCGGTAAATGCGTCGATGTCTTTGTGATGCGCGCGACATTTTTG13820.11170736872565495No Hit
CTACAAAAATGTCGCGCGCATCACAAAGACATCGACGCATTTACCGAACAA13590.10984827358767371No Hit
CAAAAATGTCGCGCGCATCACAAAGACATCGACGCATTTACCGAACAACTC12990.1049984601842444No Hit
GACGAAGACCAAGCAACACGGACCCGGGCACGTGGGAGGACGGTGTTGTAG12790.10338185571643466No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)